Rosas-Moreno Jeanette, Walker Christopher, Duffy Katie, Krüger Claudia, Krüger Manuela, Robinson Clare H, Pittman Jon K
Department of Earth and Environmental Sciences, School of Natural Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, UK.
School of Agriculture and Environment, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia; Royal Botanic Garden Edinburgh, 21A Inverleith Row, Edinburgh EH3 5LR, UK.
Sci Total Environ. 2023 Jun 10;876:162781. doi: 10.1016/j.scitotenv.2023.162781. Epub 2023 Mar 10.
Arbuscular mycorrhizal fungi were recovered from soil samples from the naturally radioactive soil at the long-abandoned South Terras uranium mine in Cornwall, UK. Species of Rhizophagus, Claroideoglomus, Paraglomus, Septoglomus, and Ambispora were recovered, and pot cultures from all except Ambispora were established. Cultures were identified to species level using morphological observation and rRNA gene sequencing combined with phylogenetic analysis. These cultures were used in pot experiments designed with a compartmentalised system to assess the contribution of fungal hyphae to the accumulation of essential elements, such as copper and zinc, and non-essential elements, such as lead, arsenic, thorium, and uranium into root and shoot tissues of Plantago lanceolata. The results indicated that none of the treatments had any positive or negative impact on shoot and root biomass. However, Rhizophagus irregularis treatments showed higher accumulation of copper and zinc in shoots, while R. irregularis and Septoglomus constrictum enhanced arsenic accumulation in roots. Moreover, R. irregularis increased uranium concentration in roots and shoots of the P. lanceolata plant. This study provides useful insight into fungal-plant interactions that determine metal and radionuclide transfer from soil into the biosphere at contaminated sites such as mine workings.
从英国康沃尔郡长期废弃的南特拉什铀矿的天然放射性土壤的土壤样本中分离出了丛枝菌根真菌。分离出了根内球囊霉属、明球囊霉属、类球囊霉属、隔球囊霉属和双孢无梗囊霉属的物种，并建立了除双孢无梗囊霉属外所有属的盆栽培养物。通过形态学观察、rRNA基因测序结合系统发育分析将培养物鉴定到种水平。这些培养物用于采用分隔系统设计的盆栽实验，以评估真菌菌丝对必需元素（如铜和锌）以及非必需元素（如铅、砷、钍和铀）在披针叶车前根和地上组织中积累的贡献。结果表明，所有处理对地上和根生物量均无任何正面或负面影响。然而，不规则根内球囊霉处理使地上部分的铜和锌积累量更高，而不规则根内球囊霉和收缩隔球囊霉增强了根中砷的积累。此外，不规则根内球囊霉增加了披针叶车前植物根和地上部分的铀浓度。这项研究为真菌 - 植物相互作用提供了有用的见解，这种相互作用决定了在诸如矿井作业等受污染场地中金属和放射性核素从土壤向生物圈的转移。
