Wang Ya-Nan, Yu Lu, Wang Ting, Liu Shiyue
Department of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong 250012, China.
Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong 250012, China.
Int J Biol Macromol. 2023 May 1;236:123998. doi: 10.1016/j.ijbiomac.2023.123998. Epub 2023 Mar 10.
Apolipoprotein E (ApoE), a protein closely related to various metabolic diseases, is recently considered to play an essential role in bone metabolism. However, the effect and mechanism of ApoE on implant osseointegration have not been clarified. This study aims to investigate the influence of additional ApoE supplementation in regulating the osteogenesis-lipogenesis balance on bone marrow mesenchymal stem cells (BMMSCs) cultured on titanium surface, and the effect of ApoE on the osseointegration of titanium implants. In vivo, the bone volume/total volume (BV/TV) and the bone-implant contact (BIC) significantly elevated in the exogenous supplement of ApoE group, compared with the Normal group. Meanwhile, the adipocyte area proportion around the implant dramatically decreased after 4-week healing. In vitro, the additional ApoE substantially drove the osteogenic differentiation of BMMSCs cultured on the titanium surface and inhibit their lipogenic differentiation as well as lipid droplet accumulation. These results suggest that ApoE, by mediating the differentiation of stem cells on the surface of titanium with this macromolecular protein, is deeply involved in facilitating titanium implant osseointegration, which reveals the potential mechanism and proposes a promising solution for further improving the osseointegration of titanium implants.
载脂蛋白E（ApoE）是一种与多种代谢性疾病密切相关的蛋白质，最近被认为在骨代谢中起重要作用。然而，ApoE对种植体骨整合的影响及机制尚未阐明。本研究旨在探讨额外补充ApoE对钛表面培养的骨髓间充质干细胞（BMMSCs）成骨-脂肪生成平衡的调节作用，以及ApoE对钛种植体骨整合的影响。在体内，与正常组相比，外源性补充ApoE组的骨体积/总体积（BV/TV）和骨-种植体接触（BIC）显著升高。同时，愈合4周后，种植体周围脂肪细胞面积比例显著降低。在体外，额外补充ApoE显著促进钛表面培养的BMMSCs的成骨分化，并抑制其脂肪生成分化以及脂滴积累。这些结果表明，ApoE通过这种大分子蛋白介导钛表面干细胞的分化，深度参与促进钛种植体骨整合，揭示了潜在机制，并为进一步改善钛种植体骨整合提出了一个有前景的解决方案。
