Chen Lei, Xiang Ming, Wu Fen, Jiang Yun, Wu Qingxi, Zhang Wenna, Guo Wenqiang, Cai Bolin, Liang Li, Li Songnan, Chen Yan, Du Xianfeng
Anhui Key Laboratory of Ecological Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, China.
State Key Lab of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Int J Biol Macromol. 2023 May 31;238:123993. doi: 10.1016/j.ijbiomac.2023.123993. Epub 2023 Mar 10.
In this study, lycopene-loaded nanofibers were successfully fabricated by electrospinning of oil-in-water (O/W) emulsions stabilized by whey protein isolate-polysaccharide TLH-3 (WPI-TLH-3) complexes. The lycopene encapsulated in the emulsion-based nanofibers exhibited enhanced photostability and thermostability, and achieved improved targeted small intestine-specific release. The release of lycopene from the nanofibers followed Fickian diffusion mechanism in simulated gastric fluid (SGF) and first-order model in simulated intestinal fluid (SIF) with the enhanced release rates. The bioaccessibility and cellular uptake efficiency of lycopene in micelles by Caco-2 cells after in vitro digestion were significantly improved. The intestinal membrane permeability and transmembrane transport efficiency of lycopene in micelles across Caco-2 cells monolayer were greatly elevated, thus promoting the effective absorption and intracellular antioxidant activity of lycopene. This work opens a potential approach for electrospinning of emulsions stabilized by protein-polysaccharide complexes as a novel delivery system for liposoluble nutrients with enhanced bioavailability in functional food industries.
在本研究中，通过静电纺丝法，以乳清蛋白分离物 - 多糖TLH - 3（WPI - TLH - 3）复合物稳定的水包油（O/W）乳液成功制备了负载番茄红素的纳米纤维。包封在乳液基纳米纤维中的番茄红素表现出增强的光稳定性和热稳定性，并实现了改善的靶向小肠特异性释放。番茄红素从纳米纤维中的释放在模拟胃液（SGF）中遵循菲克扩散机制，在模拟肠液（SIF）中遵循一级模型，且释放速率有所提高。体外消化后，Caco - 2细胞对番茄红素在胶束中的生物可及性和细胞摄取效率显著提高。番茄红素在胶束中跨Caco - 2细胞单层的肠膜通透性和跨膜转运效率大大提高，从而促进了番茄红素的有效吸收和细胞内抗氧化活性。这项工作为通过蛋白质 - 多糖复合物稳定的乳液进行静电纺丝开辟了一种潜在方法，作为功能食品工业中具有增强生物利用度的脂溶性营养物质的新型递送系统。
