Faculty of Engineering, Computing and Science, Swinburne University of Technology Sarawak, 93350, Kuching, Malaysia.
School of Science, Engineering and Technology, Abertay University, Dundee, DD1 1HG, UK.
Sci Rep. 2023 Mar 12;13(1):4095. doi: 10.1038/s41598-023-31197-4.
Early Mortality Syndrome (EMS) has been a major problem for shrimp aquaculture in Southeast Asia due to its epizootic prevalence within the region since the first reported case in 2009. This study explores the application of halophilic marine bacilli isolated from coral mucus and their quorum-quenching abilities as potential biocontrol agents in aquaculture systems to combat the causative agent of EMS, Vibrio parahaemolyticus. N-acylhomoserine lactone (AHL)-degrading (AiiA) activity was first screened by PCR then confirmed by bio-reporter assay, and a combination of 16S rDNA sequence analysis and quantitative phenotype assays including biofilm-formation and temperature-growth responses were used to demonstrate diversity amongst these quorum-quenching isolates. Three phenotypically distinct strains showing notable potential were chosen to undergo co-cultivation as a method for strain improvement via long term exposure to the pathogenic V. parahaemolyticus. The novel approach taken led to significant improvements in antagonism and quorum quenching activities as compared to the ancestral wild-type strains and offers a potential solution as well as pathway to improve existing beneficial microbes for one of the most pressing issues in shrimp aquacultures worldwide.
早期死亡综合征（EMS）一直是东南亚虾类养殖业的一个主要问题，因为自 2009 年首例报告以来，该疾病在该地区流行。本研究探讨了从珊瑚黏液中分离出的嗜盐海洋杆菌及其群体感应淬灭能力作为水产养殖系统中对抗 EMS 病原体副溶血弧菌的潜在生物防治剂的应用。首先通过 PCR 筛选 N-酰基高丝氨酸内酯 (AHL) 降解（AiiA）活性，然后通过生物报告测定进行确认，并结合 16S rDNA 序列分析和包括生物膜形成和温度生长响应在内的定量表型测定来证明这些群体感应淬灭分离株之间的多样性。选择三种表型明显不同且具有显著潜力的菌株进行共培养，作为通过长期暴露于致病性副溶血弧菌来改善菌株的方法。与原始野生型菌株相比，这种新方法显著提高了拮抗作用和群体感应淬灭活性，为解决全球虾类养殖业中最紧迫的问题之一提供了一种潜在的解决方案和途径，即改善现有的有益微生物。
