Adak Totan, Mahanty Arabinda, Sarkar Sutapa, Basak Nabaneeta, Kumar Gaurav, Sanghamitra Priyadarsini, Bagchi Torit Baran, Chakraborti Mridul
ICAR-National Rice Research Institute, Cuttack, Odisha 753006 India.
J Food Sci Technol. 2023 Mar;60(3):1185-1194. doi: 10.1007/s13197-023-05674-7. Epub 2023 Feb 3.
The commercial significance of accurate and simple quantification of 2-Acetyl-1-pyrroline (2-AP) cannot be overstated. Present study was carried out to standardize a method for extraction and accurate quantitation of 2-AP from rice grain using GC-MS/MS equipped with HS-SPME auto sampler. The effect of sample quantity, addition of solvent, grinding process, sample particle size, head space parameters and SPME fiber incubation parameters, were optimized in the developed method. Dehusked rice powder (2 g) prepared under liquid nitrogen, and passed through the 80-mesh sieve, incubated for 40 min at 80 °C in headspace, followed by fiber (DVB/Carbon WR/PDMS) saturation time of 15 min, could produce the maximum response. The recovery of 2-AP from fortified sample ranged between 7.02 and 9.02% at 50-200 ng g fortification irrespective of the grain matrices used. Standard addition method was appropriate to overcome the matrix effect and recovery of 2-AP was more than 90% using this method. The developed method was further utilized for quantification of 2-AP in four Basmati and two non-Basmati aromatic rice samples. The content of 2-AP ranged between 57.17 and 147.10 ng g of rice and varied with geographical location. This fully automated method could improve the work efficiency and reduce error during the volatile extraction and adsorption phase.
The online version contains supplementary material available at 10.1007/s13197-023-05674-7.
准确且简单地定量测定2-乙酰基-1-吡咯啉（2-AP）的商业意义再怎么强调也不为过。本研究旨在标准化一种使用配备顶空固相微萃取自动进样器的气相色谱-串联质谱法从稻谷中提取和准确定量2-AP的方法。在已开发的方法中，对样品量、溶剂添加、研磨过程、样品粒度、顶空参数和固相微萃取纤维孵育参数的影响进行了优化。在液氮条件下制备的2克糙米粉末，通过80目筛，在顶空80℃下孵育40分钟，随后纤维（二乙烯基苯/碳WR/聚二甲基硅氧烷）饱和时间为15分钟，可产生最大响应。无论使用何种谷物基质，在50-200纳克/克的加标水平下，加标样品中2-AP的回收率在7.02%至9.02%之间。标准加入法适用于克服基质效应，使用该方法2-AP的回收率超过90%。已开发的方法进一步用于定量四个巴斯马蒂香米和两个非巴斯马蒂香米样品中的2-AP。2-AP的含量在每克大米57.17至147.10纳克之间，且因地理位置而异。这种全自动方法可以提高工作效率，并减少挥发性物质提取和吸附阶段的误差。
在线版本包含可在10.1007/s13197-023-05674-7获取的补充材料。
