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大鼠雪旺细胞体外分化与去分化体系的建立

Establishment of an in vitro Differentiation and Dedifferentiation System of Rat Schwann Cells.

作者信息

Zou Ying

机构信息

Biology of Postdoctoral Research Station, Guangdong Second Provincial General Hospital, School of Medicine, Jinan University, Guangzhou, China.

出版信息

Bio Protoc. 2023 Mar 5;13(5):e4631. doi: 10.21769/BioProtoc.4631.

DOI:10.21769/BioProtoc.4631
PMID:36908636
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9993079/
Abstract

In the peripheral nervous system, Schwann cells are the primary type of glia; their in vitro differentiation and dedifferentiation system has not been described in detail in the literature. Thus, an in vitro differentiation and dedifferentiation system of rat Schwann cells is described in this protocol. These cultures and systems may be used to investigate the morphological and biochemical effects of drug interventions or lentivirus-mediated gene transfer on Schwann cells during differentiation or dedifferentiation. Graphical abstract.

摘要

在周围神经系统中，施万细胞是主要的神经胶质细胞类型；其体外分化和去分化系统在文献中尚未得到详细描述。因此，本方案描述了大鼠施万细胞的体外分化和去分化系统。这些培养物和系统可用于研究药物干预或慢病毒介导的基因转移在施万细胞分化或去分化过程中对其形态和生化的影响。图形摘要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/f948cab32a76/BioProtoc-13-05-4631-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/ba55d372fb32/BioProtoc-13-05-4631-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/2c1a3b2f2876/BioProtoc-13-05-4631-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/5256380ccbbc/BioProtoc-13-05-4631-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/f948cab32a76/BioProtoc-13-05-4631-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/ba55d372fb32/BioProtoc-13-05-4631-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/2c1a3b2f2876/BioProtoc-13-05-4631-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/5256380ccbbc/BioProtoc-13-05-4631-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b7f/9993079/f948cab32a76/BioProtoc-13-05-4631-g004.jpg

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Establishment of an in vitro Differentiation and Dedifferentiation System of Rat Schwann Cells.大鼠雪旺细胞体外分化与去分化体系的建立
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本文引用的文献

1
Development of myelinating glia: An overview.成髓鞘胶质细胞的发育：概述。
Glia. 2022 Dec;70(12):2237-2259. doi: 10.1002/glia.24238. Epub 2022 Jul 4.
2
SIRT6 Negatively Regulates Schwann Cells Dedifferentiation via Targeting c-Jun During Wallerian Degeneration After Peripheral Nerve Injury.SIRT6在周围神经损伤后的沃勒变性过程中通过靶向c-Jun负向调节雪旺细胞去分化。
Mol Neurobiol. 2022 Jan;59(1):429-444. doi: 10.1007/s12035-021-02607-3. Epub 2021 Oct 27.
3
Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat.
新生大鼠脊神经雪旺细胞的分离与纯化
Bio Protoc. 2017 Oct 20;7(20):e2588. doi: 10.21769/BioProtoc.2588.
4
Mechanisms of Schwann cell plasticity involved in peripheral nerve repair after injury.损伤后周围神经修复中施万细胞可塑性的机制。
Cell Mol Life Sci. 2020 Oct;77(20):3977-3989. doi: 10.1007/s00018-020-03516-9. Epub 2020 Apr 10.
5
The Success and Failure of the Schwann Cell Response to Nerve Injury.施万细胞对神经损伤反应的成功与失败
Front Cell Neurosci. 2019 Feb 11;13:33. doi: 10.3389/fncel.2019.00033. eCollection 2019.
6
The atypical Guanine-nucleotide exchange factor, dock7, negatively regulates schwann cell differentiation and myelination.非典型鸟嘌呤核苷酸交换因子 Dock7 负调控许旺细胞分化和髓鞘形成。
J Neurosci. 2011 Aug 31;31(35):12579-92. doi: 10.1523/JNEUROSCI.2738-11.2011.
7
Mouse schwann cells need both NRG1 and cyclic AMP to myelinate.施万细胞需要 NRG1 和环 AMP 来髓鞘化。
Glia. 2011 May;59(5):720-33. doi: 10.1002/glia.21144. Epub 2011 Feb 14.