Angelucci Alessandra, Clark Andrew, Ingold Alexander, Reiche Christopher, Cundy Donald, Balsor Justin, Federer Frederick, McAlinden Niall, Cheng Yunzhou, Rolston John, Rieth Loren, Dawson Martin, Mathieson Keith, Blair Steve
Moran Eye Institute, University of Utah Health.
University of Utah.
Res Sq. 2023 Feb 28:rs.3.rs-2322768. doi: 10.21203/rs.3.rs-2322768/v1.
Optogenetics has transformed studies of neural circuit function, but remains challenging to apply in non-human primates (NHPs). A major challenge is delivering intense and spatially precise patterned photostimulation across large volumes in deep tissue. Here, we have developed and validated the Utah Optrode Array (UOA) to meet this critical need. The UOA is a 10×10 glass waveguide array bonded to an electrically-addressable μLED array. electrophysiology and immediate early gene (c-fos) immunohistochemistry demonstrated the UOA allows for large-scale spatiotemporally precise neuromodulation of deep tissue in macaque primary visual cortex. Specifically, the UOA permits both focal (single layers or columns), and large-scale (across multiple layers or columns) photostimulation of deep cortical layers, simply by varying the number of simultaneously activated μLEDs and/or the light irradiance. These results establish the UOA as a powerful tool for studying targeted neural populations within single or across multiple deep layers in complex NHP circuits.
光遗传学已经改变了神经回路功能的研究，但在非人类灵长类动物（NHPs）中应用仍然具有挑战性。一个主要挑战是在深部组织的大体积范围内进行强烈且空间精确的图案化光刺激。在这里，我们开发并验证了犹他光电极阵列（UOA）以满足这一关键需求。UOA是一个10×10的玻璃波导阵列，与一个电寻址μLED阵列相连。电生理学和即刻早期基因（c-fos）免疫组织化学表明，UOA能够对猕猴初级视觉皮层的深部组织进行大规模的时空精确神经调节。具体而言，UOA只需通过改变同时激活的μLED数量和/或光辐照度，就可以对深部皮层层进行局部（单层或单列）和大规模（跨多层或多列）的光刺激。这些结果确立了UOA作为研究复杂非人类灵长类动物回路中单个或多个深层内靶向神经群体的强大工具。
