Adamczak Rafał, Ukleja-Sokołowska Natalia, Lis Kinga, Bartuzi Zbigniew, Dubiel Mariusz
Department of Obstetrics and Gynecology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland.
Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland.
Postepy Dermatol Alergol. 2023 Feb;40(1):119-125. doi: 10.5114/ada.2022.124304. Epub 2023 Jan 19.
Matrix metalloproteinases (MMP) degrade extracellular matrix. Some studies show that MMP9 concentration in follicular fluid (FF) may play a role in oocyte maturation and fertilization (IVF) success. The immunology of follicular fluid is still not fully understood.
Assessment of RANTES, MIP4A, MMP7, MMP9, MMP14, TIMP 1, TIMP 2 and TIMP 3 concentration in the follicular fluid of patients undergoing fertilization/embryo transfer procedure.
This case-control study included 20 randomly selected patients with a positive pregnancy (PPG) test and 20 patients with a negative pregnancy (NPG) test after IVF/ET. In FF obtained during oocyte retrieval, the concentrations of MIP4A, MMP7, MMP14, TIMP 1, TIMP 2, TIMP 3, RANTES, IL-12p40, and IL-17A were measured. Their effect on the characteristics of follicles, embryos, and the efficiency of IVF and ET were analysed.
There was no statistically significant relationship between a positive pregnancy test and the results of the immunoassay performed. The number of COC-1 correlates significantly and positively with RANTES ( = 0.34; = 0.038) and IP-10 ( = 0.329; = 0.038). MII correlates significantly and positively with RANTES ( = 0.341, = 0.031). The number of top-quality embryos correlates significantly and positively with IL-17A ( = 0.451, = 0.004) and TIMP 1 ( = 0.44, = 0.005).
The concentration of IL-17A and TIMP 1 may predict IVF/ET success. Further studies are required on the influence of the follicular fluid immunological environment on oocyte maturation and quality and, subsequently, embryo development.
基质金属蛋白酶（MMP）可降解细胞外基质。一些研究表明，卵泡液（FF）中的MMP9浓度可能在卵母细胞成熟和受精（体外受精）成功率方面发挥作用。卵泡液的免疫学仍未被完全了解。
评估接受受精/胚胎移植手术患者卵泡液中调节激活正常T细胞表达和分泌的趋化因子（RANTES）、巨噬细胞炎性蛋白4A（MIP4A）、基质金属蛋白酶7（MMP7）、基质金属蛋白酶9（MMP9）、基质金属蛋白酶14（MMP14）、金属蛋白酶组织抑制因子1（TIMP 1）、金属蛋白酶组织抑制因子2（TIMP 2）和金属蛋白酶组织抑制因子3（TIMP 3）的浓度。
本病例对照研究纳入了20例体外受精/胚胎移植术后妊娠试验呈阳性（PPG）的随机选择患者和20例妊娠试验呈阴性（NPG）的患者。在取卵过程中获得的卵泡液中，测量了MIP4A、MMP7、MMP14、TIMP 1、TIMP 2、TIMP 3、RANTES、白细胞介素12 p40（IL-12p40）和白细胞介素17A（IL-17A）的浓度。分析了它们对卵泡、胚胎特征以及体外受精和胚胎移植效率的影响。
妊娠试验呈阳性与所进行的免疫测定结果之间无统计学显著关系。卵丘-卵母细胞复合体1（COC-1）的数量与RANTES（r = 0.34；P = 0.038）和干扰素γ诱导蛋白10（IP-10）（r = 0.329；P = 0.038）显著正相关。第二次减数分裂中期（MII）与RANTES显著正相关（r = 0.341，P = 0.031）。优质胚胎的数量与IL-17A（r = 0.451，P = 0.004）和TIMP 1（r = 0.44，P = 0.005）显著正相关。
IL-17A和TIMP 1的浓度可能预测体外受精/胚胎移植的成功率。需要进一步研究卵泡液免疫环境对卵母细胞成熟和质量以及随后胚胎发育的影响。
