Kim Sungho, Lee Chang Min, Lee Yoontaek, Han Hyung-Joon, Song Tae-Jin
Department of Surgery, Korea University Ansan Hospital, Ansan, Korea.
Department of Surgery, Korea University College of Medicine, Seoul, Korea.
Ann Surg Treat Res. 2023 Mar;104(3):144-149. doi: 10.4174/astr.2023.104.3.144. Epub 2023 Feb 28.
Near-infrared fluorescence imaging has been recently applied in the field of hepatobiliary surgery. Our objective was to apply blue-light fluorescence cholangiography during laparoscopic surgery. Therefore, we designed a preclinical study to evaluate the feasibility of using blue-light fluorescence for cholangiography in a porcine model.
Five millimeters of sodium fluorescein (SF) solution was administered into the gallbladder of 20 male 3-way crossbred (Landrace × Yorkshire × Duroc) pigs in laparoscopic approach. The biliary tree was observed under blue light (a peak wavelength of 450 nm) emitted from a commercialized light-emitting diode (LED) light source (XLS1 extreme, Chammed).
In 18 of 20 porcine models, immediately after SF solution was administered into the gallbladder, it was possible to visualize the biliary tree under blue light emitted from the LED light source.
This study provided a preclinical basis for using blue-light fluorescence cholangiography using SF in laparoscopic surgery. The clinical feasibility of blue-light fluorescence imaging techniques for laparoscopic cholecystectomy remained to be demonstrated.
近红外荧光成像最近已应用于肝胆外科领域。我们的目标是在腹腔镜手术中应用蓝光荧光胆管造影术。因此，我们设计了一项临床前研究，以评估在猪模型中使用蓝光荧光进行胆管造影的可行性。
通过腹腔镜手术将5毫米的荧光素钠（SF）溶液注入20头雄性三元杂交（长白猪×约克夏猪×杜洛克猪）猪的胆囊中。使用商业化发光二极管（LED）光源（XLS1 extreme，Chammed）发出的蓝光（峰值波长450纳米）观察胆管树。
在20个猪模型中的18个中，在将SF溶液注入胆囊后，立即可以在LED光源发出的蓝光下看到胆管树。
本研究为在腹腔镜手术中使用SF进行蓝光荧光胆管造影提供了临床前依据。蓝光荧光成像技术用于腹腔镜胆囊切除术的临床可行性仍有待证明。
