Shen Yanjun, Li Qinghua, Cheng Ruli, Luo Yang, Zhang Yufeng, Zuo Qing
Laboratory of Water Ecological Health and Environmental Safety, School of Life Sciences Chongqing Normal University Chongqing China.
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences Southwest University Chongqing China.
Ecol Evol. 2023 Mar 8;13(3):e9858. doi: 10.1002/ece3.9858. eCollection 2023 Mar.
As an endemic freshwater crab group in China, the phylogenetic relationships within are still controversial because of the limited taxon samples. In this study, the complete mitogenomes of with 17,311 bp and the nearly complete mitogenomes of with 16,785 bp were firstly sequenced and analyzed. Compared with other reported mitogenomes of Potamidae, some novel patterns of gene rearrangement were detected in these two mitogenomes, which could be illuminated by the mechanisms of tandem duplication-random loss, recombination, and translocation. Phylogenetic analyses showed the nonmonophyly of the and a sister group relationship with . These crabs from the eastern and southern of the Yangtze River basin were more closely related while other crabs form the plateau areas formed a separate clade. Divergence time indicated that the and its sister group diverged from other potamiscine freshwater crabs approximately 42.65 Mya, which belongs to the recent main uplifts period of the Tibetan Plateau in the Late Miocene. Combined with the similar evolutionary rates and relatively stable habitat altitude of these species, these results implied that the ecological environment may be relatively stable during the speciation. Overall, our study yielded worthy perceptions for the evolutionary and taxonomic relationship of and will help to better clarify the gene rearrangement events of the invertebrate mitogenome and lay the foundation for further phylogenetic study of . Overall, our study yielded valuable insights into the evolutionary history and taxonomic relationship of and these results will help to better explain the gene rearrangement events of the invertebrate mitogenome and lay the foundation for further phylogenetic study of .
作为中国特有的淡水蟹类群，由于分类单元样本有限，其内部的系统发育关系仍存在争议。在本研究中，首次对长度为17,311 bp的[蟹种名称1]的完整线粒体基因组和长度为16,785 bp的[蟹种名称2]的近乎完整的线粒体基因组进行了测序和分析。与其他已报道的溪蟹科线粒体基因组相比，在这两个[蟹种名称]的线粒体基因组中检测到了一些新的基因重排模式，这些模式可以通过串联重复-随机丢失、重组和易位机制来解释。系统发育分析表明[蟹种名称1]并非单系类群，且与[蟹种名称2]形成姐妹群关系。长江流域东部和南部的这些蟹类关系更为密切，而来自高原地区的其他蟹类则形成一个单独的分支。分歧时间表明[蟹种名称1]及其姐妹群[蟹种名称2]大约在4265万年前与其他溪蟹科淡水蟹类分化，这一时期属于晚中新世青藏高原最近的主要隆升期。结合这些[蟹种名称]物种相似的进化速率和相对稳定的栖息地海拔，这些结果表明在物种形成过程中生态环境可能相对稳定。总体而言，我们的研究为[蟹种名称]的进化和分类关系提供了有价值的见解，将有助于更好地阐明无脊椎动物线粒体基因组的基因重排事件，并为进一步对[蟹种名称]进行系统发育研究奠定基础。总体而言，我们的研究对[蟹种名称]的进化历史和分类关系产生了有价值的见解，这些结果将有助于更好地解释无脊椎动物线粒体基因组的基因重排事件，并为进一步对[蟹种名称]进行系统发育研究奠定基础。
