College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, PR China.
Yunnan Dong Heng Economic and Trade Group Co., Ltd., Qujing 655000, Yunnan, PR China.
Food Res Int. 2023 Apr;166:112610. doi: 10.1016/j.foodres.2023.112610. Epub 2023 Feb 17.
The quality of hams obtained from different pig breeds can vary depending on endogenous antioxidant peptides in the hams. The aims of this study were (i) to investigate the specific peptides in Chinese Dahe black pig ham (DWH) and hybrid pig ham (Yorkshire × Landrace × Dahe black ham, YLDWH) and their antioxidant activity, and (ii) to elucidate the relationship between ham quality and antioxidant peptides. iTRAQ quantitative peptidomic method was used to discover specific peptides of DWH and YLDWH. In addition, in vitro assays were performed to evaluate their antioxidant activity. A total of 73 specific peptides were identified from DWH and YLDWH by LC-MS/MS. Forty-four specific peptides in DWH were primarily hydrolysed from myosin and myoglobin by endopeptidases, while 29 specific peptides in YLDWH were primarily hydrolysed from myosin and troponin-T. Six specific peptides that were statistically significantly different based on their fold changes and P-values were selected for the identification of DWH and YLDWH. DWH-derived specific peptide AGAPDERGPGPAAR (AR14), which had high stability and was non-toxic, had the highest DPPH• and ABTS•+ scavenging activity (IC = 1.657 mg/mL and 0.173 mg/mL, respectively) and cellular antioxidant capacity. Molecular docking showed that AR14 interacted with Val369, and Val420 of Keap1 via hydrogen bonds. Furthermore, AR14 bound to DPPH and ABTS through hydrogen bonding and hydrophobic interactions. Together, our results demonstrate that the DWH-derived antioxidant peptide AR14 exhibits the free radical scavenging and cellular antioxidant activity and can be used to preserve ham quality and promote human health.
不同猪种获得的火腿质量可能因火腿中的内源性抗氧化肽而异。本研究的目的是：（i）研究中国大河黑猪火腿（DWH）和杂交猪火腿（约克夏猪×长白猪×大河黑猪，YLDWH）中的特定肽及其抗氧化活性；（ii）阐明火腿质量与抗氧化肽之间的关系。使用 iTRAQ 定量肽组学方法发现 DWH 和 YLDWH 的特定肽。此外，还进行了体外测定来评估它们的抗氧化活性。通过 LC-MS/MS 从 DWH 和 YLDWH 中鉴定出 73 种特定肽。DWH 中的 44 种特定肽主要由内肽酶从肌球蛋白和肌红蛋白中水解而来，而 YLDWH 中的 29 种特定肽主要由肌球蛋白和肌钙蛋白-T 中水解而来。根据它们的变化倍数和 P 值，选择了六个在统计学上有显著差异的特定肽来鉴定 DWH 和 YLDWH。DWH 衍生的特定肽 AGAPDERGPGPAAR（AR14）具有较高的稳定性和低毒性，其 DPPH•和 ABTS•+清除活性（IC=1.657mg/mL 和 0.173mg/mL）和细胞抗氧化能力最高。分子对接表明，AR14 通过氢键与 Keap1 的 Val369 和 Val420 相互作用。此外，AR14 通过氢键和疏水相互作用与 DPPH 和 ABTS 结合。总之，我们的结果表明，DWH 衍生的抗氧化肽 AR14 具有清除自由基和细胞抗氧化活性，可以用于保持火腿质量和促进人类健康。
