Universidad Autónoma Chapingo - Departamento de Fitotecnia, Km. 38.5, Carretera México-Texcoco, 56230 Chapingo, Estado de México, Mexico.
Universidad Autónoma Chapingo - Departamento de Fitotecnia, Km. 38.5, Carretera México-Texcoco, 56230 Chapingo, Estado de México, Mexico.
Food Res Int. 2023 Apr;166:112489. doi: 10.1016/j.foodres.2023.112489. Epub 2023 Jan 20.
Purslane (Portulaca oleracea L.) has a high content of nutrients and medicinal effects that depend on the genotype, harvesting time, and production system. The objective of the present research work was to elucidate the NMR-based metabolomics profiling of three native purslane cultivars from Mexico (Xochimilco, Mixquic, and Cuautla) grown under hydroponic conditions and harvested in three different times (32, 39, and 46 days after emergence). Thirty-nine metabolites identified in the H NMR spectra of aerial parts of purslane, 5 sugars, 15 amino acids, 8 organic acids, 3 caffeoylquinic acids, as well as 2 alcohols and 3 nucleosides, choline, O-phosphocholine and trigonelline were also detected. A total of 37 compounds were detected in native purslane from Xochimilco and Cuautla, whereas 39 compounds were detected in purslane from Mixquic. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) separated the cultivars into three clusters. Mixquic cultivar had the highest number of differential compounds (amino acids and carbohydrates), followed by Xochimilco and Cuautla cultivars, respectively. Changes in the metabolome were observed in latest times of harvest for all the cultivars studied. The differential compounds were glucose, fructose, galactose, pyruvate, choline, and 2-hydroxysobutyrate. The results obtained in this investigation may contribute to selecting the best cultivar of purslane and the best time in which the levels of nutrients are optimal.
马齿苋（Portulaca oleracea L.）含有丰富的营养成分和药用功效，这些成分取决于基因型、收获时间和生产系统。本研究工作的目的是阐明在水培条件下生长并在不同时间（出苗后 32、39 和 46 天）收获的三种墨西哥本地马齿苋品种（霍奇米尔科、米克斯奎克和夸特利亚）基于 NMR 的代谢组学图谱。在马齿苋地上部分的 H NMR 图谱中鉴定出 39 种代谢物，包括 5 种糖、15 种氨基酸、8 种有机酸、3 种咖啡酰奎宁酸以及 2 种醇和 3 种核苷、胆碱、O-磷酸胆碱和尿嘧啶核苷。在霍奇米尔科和夸特利亚的本地马齿苋中检测到 37 种化合物，而在米克斯奎克的马齿苋中检测到 39 种化合物。主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）将品种分为三个聚类。米克斯奎克品种的差异化合物（氨基酸和碳水化合物）数量最多，其次是霍奇米尔科和夸特利亚品种。所有研究品种的收获后期都观察到代谢组的变化。差异化合物为葡萄糖、果糖、半乳糖、丙酮酸、胆碱和 2-羟基丁酸盐。本研究获得的结果可能有助于选择最佳的马齿苋品种和营养成分最佳的收获时间。
