Finch G L, McNeill K L, Hayes T L, Fisher G L
Donner Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Environ Res. 1987 Dec;44(2):241-53. doi: 10.1016/s0013-9351(87)80233-5.
Pulmonary alveolar macrophage cells (PAM) are an important component of the pulmonary response to particles deposited in the deep lung. To more fully characterize the interactions between macrophages and particulate materials, a correlative light and electron microscopic technique was developed that allowed light microscopic identification of individual cell viability after in vitro particle exposure, followed by scanning and transmission electron microscopic analyses of specific PAM, including surface morphology, X-ray microanalytic evaluation of particle content, and internal cellular structure. Individual cell viability, particle content, and morphologic alterations were evaluated for three particle types: Ni3S2, TiO2, and glass beads. Cell death and stages of cell disruption including bleb cluster formation, loss of surface features, formation of membrane tears and holes, and cell degranulation correlated with Ni3S2 and TiO2 content. Glass beads were not associated with cell disruption or viability reduction. Correlative microscopic techniques were essential in describing particle-dependent effects on an individual cell basis.
肺泡巨噬细胞（PAM）是肺部对沉积在肺深部颗粒作出反应的重要组成部分。为了更全面地表征巨噬细胞与颗粒物质之间的相互作用，开发了一种相关光镜和电镜技术，该技术能够在体外颗粒暴露后通过光镜鉴定单个细胞的活力，随后对特定的肺泡巨噬细胞进行扫描和透射电镜分析，包括表面形态、颗粒含量的X射线微分析评估以及内部细胞结构。对三种颗粒类型进行了单个细胞活力、颗粒含量和形态学改变的评估：Ni3S2、TiO2和玻璃珠。细胞死亡以及细胞破坏的阶段，包括泡状簇形成、表面特征丧失、膜撕裂和孔洞形成以及细胞脱颗粒，与Ni3S2和TiO2含量相关。玻璃珠与细胞破坏或活力降低无关。相关显微镜技术对于在单个细胞基础上描述颗粒依赖性效应至关重要。
