Pasquini R, Scassellati Sforzolini G, Savino A, Angeli G, Monarca S
Department of Hygiene, University of Perugia, Italy.
Environ Res. 1987 Dec;44(2):302-11. doi: 10.1016/s0013-9351(87)80239-6.
Aryl hydrocarbon hydroxylase (AHH) and dimethylnitrosamine demethylase (DMND) activities in pulmonary and hepatic tissues of male Sprague-Dawley rats were assayed following pretreatment with known inducers (benzo[a]pyrene, 3-methylcholanthrene, Aroclor 1254, phenobarbital) and with main-stream (MS) and side-stream (SS) cigarette smoke condensates and their related fractions. Biochemical assays by spectrophotofluorimetry (AHH activity) and spectrophotometry (DMND activity) and by a biological assay (Ames test) were performed to detect AHH and DMND induction. Ames test proved to be much less sensitive than the spectrophotofluorimetric analysis for AHH determination. Both main-stream and side-stream cigarette smoke condensates and some fractions, containing water-soluble bases, water-insoluble bases, and polycyclic aromatic hydrocarbons, were found to induce AHH activity in lung and liver, the lung being induced to the greatest extent. The highest levels of AHH inducibility were found for the SS-smoke condensate and related fractions. In particular, the insoluble bases fractions gave the highest induction. On the contrary, pulmonary DMND activity was not affected by pretreatment with the same materials, while hepatic DMND response was only minimally induced by Aroclor and phenobarbital treatment.
在用已知诱导剂（苯并[a]芘、3-甲基胆蒽、多氯联苯混合物1254、苯巴比妥）以及主流（MS）和侧流（SS）香烟烟雾浓缩物及其相关组分进行预处理后，对雄性斯普拉格-道利大鼠的肺和肝组织中的芳烃羟化酶（AHH）和二甲基亚硝胺脱甲基酶（DMND）活性进行了测定。通过分光荧光法（AHH活性）和分光光度法（DMND活性）以及生物测定法（艾姆斯试验）进行生化测定，以检测AHH和DMND的诱导情况。结果证明，艾姆斯试验对AHH测定的敏感性远低于分光荧光分析。发现主流和侧流香烟烟雾浓缩物以及一些含有水溶性碱、水不溶性碱和多环芳烃的组分均可诱导肺和肝中的AHH活性，其中肺的诱导程度最大。SS烟雾浓缩物及其相关组分的AHH诱导水平最高。特别是，不溶性碱组分的诱导作用最强。相反，相同物质预处理对肺DMND活性没有影响，而肝DMND反应仅在多氯联苯混合物和苯巴比妥处理时受到最小程度的诱导。
