Department of Poultry Production, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
Department of Histology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Anim Biotechnol. 2023 Dec;34(8):3647-3657. doi: 10.1080/10495398.2023.2188054. Epub 2023 Mar 13.
This study aimed to evaluate impacts of dietary probiotics PB6) and humate substances (HS) supplementation on histomorphometry of small intestine and immune organs, blood parameters of growing quail. A total of 216 unsexed quails (seven days old) were randomly distributed to six groups. The 1 group did not receive any supplements (control), 2 group received (CloSTAT: 0.5 g/kg diet), 3 and 4 groups received HS (4 and 8 g/kg diet, respectively), 5 and 6 groups received CloSTAT + 4g HS and CloSTAT + 8g HS, respectively. Results showed that the inclusion of alone in quail diets significantly improved histomorphometry indices of intestine and immune organs compared to the control. Dietary supplementation of HS alone led to deteriorating histomorphometry indices of intestinal segments and immune organs compared to the control. CloSTAT, HS or both improved lipid profile and antioxidant parameters. Serum mineral levels did not differ significantly among groups except for Ca levels. In conclusion, dietary probiotics supplementation enhanced histomorphometry of intestine and immune organs and improved serum Ca, lipid profile and antioxidant indices. Moreover, the addition of HS (4 or 8 g/kg diet) improved lipid profile and antioxidant indices, but led to undesirable results in intestinal development and immune organs.
本研究旨在评估日粮益生菌（PB6）和腐殖酸钠（HS）添加对生长鹌鹑小肠和免疫器官组织形态学以及血液参数的影响。共选用 216 只（7 日龄）非性别鹌鹑随机分为 6 组。第 1 组不添加任何补充剂（对照组），第 2 组添加 CloSTAT（0.5 g/kg 日粮），第 3 组和第 4 组分别添加 HS（4 和 8 g/kg 日粮），第 5 组和第 6 组分别添加 CloSTAT + 4g HS 和 CloSTAT + 8g HS。结果表明，与对照组相比，日粮单独添加 CloSTAT 显著改善了肠道和免疫器官的组织形态学指标。与对照组相比，单独添加 HS 会导致肠道和免疫器官的组织形态学指标恶化。CloSTAT、HS 或两者均可改善脂质谱和抗氧化参数。血清矿物质水平除 Ca 水平外，各组间无显著差异。结论：日粮添加益生菌可增强肠道和免疫器官的组织形态学，改善血清 Ca、脂质谱和抗氧化指标。此外，添加 HS（4 或 8 g/kg 日粮）可改善脂质谱和抗氧化指标，但会导致肠道发育和免疫器官出现不良结果。
