Xia Xiaodong, Wang Lijun, Sun Pengyu, Liu Yu, Liu Hongze, Li Jiao, Chen Qiang, Shangguan Wenfeng, Wang Weiding, Miao Shuai, Gu Tianshu, Liu Enzhao, Liang Xue, Li Guangping
Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, The Second Hospital of Tianjin Medical University Tianjin 300211, China.
Department of Emergency Medicine, Tianjin Medical University General Hospital Tianjin 300052, China.
Am J Transl Res. 2023 Feb 15;15(2):1041-1051. eCollection 2023.
A novel temperature-controlled intravascular radiofrequency balloon angioplasty (RFBA) technique was designed and developed for atherosclerosis (AS) management.
After establishing an AS model based on a balloon denudation injury of the abdominal aorta and a high cholesterol diet in rabbits, 46 animals were randomly assigned to the RFBA group (n = 28) or the plain balloon angioplasty (PBA) group (n = 28). The groups were further subdivided based on post-treatment euthanasia times (1 hour, 7 days, 14 days, and 28 days). Histopathological changes were observed by hematoxylin and eosin and Masson's staining. Immunohistochemistry, western blotting, and real-time quantitative polymerase chain reaction were used to detect changes in pro-inflammatory, anti-inflammatory, and apoptotic factors; TGF-β/Smad-2 pathway protein Immune levels; and mRNA levels in tissues, respectively.
The vascular lumen area in the RFBA group was larger than that in the PBA group at the same time points, although the change in the vascular lumen area was not different between groups. The expression of Bax, TGF-β, Smad-2, and Caspase-3 in the RFBA group was significantly higher than that in the PBA group. The expression levels of Bcl-2 in the RFBA group were significantly lower than those in the PBA group.
At 28 days, RFBA dilated the atherosclerotic blood vessels and thickened the fibrous cap of atherosclerotic plaques to promote plaque stability. RFBA was also found to activate apoptotic factors and the TGF-/Smad-2 inflammatory pathway.
设计并开发一种新型的温控血管内射频球囊血管成形术（RFBA）技术用于动脉粥样硬化（AS）的治疗。
在兔腹主动脉球囊剥脱损伤并给予高胆固醇饮食建立AS模型后，将46只动物随机分为RFBA组（n = 28）和平行球囊血管成形术（PBA）组（n = 28）。根据治疗后安乐死时间（1小时、7天、14天和28天）将每组进一步细分。通过苏木精-伊红染色和Masson染色观察组织病理学变化。分别采用免疫组织化学、蛋白质印迹法和实时定量聚合酶链反应检测促炎、抗炎和凋亡因子的变化；TGF-β/Smad-2信号通路蛋白免疫水平；以及组织中的mRNA水平。
在相同时间点，RFBA组的血管腔面积大于PBA组，尽管两组血管腔面积的变化无差异。RFBA组中Bax、TGF-β、Smad-2和Caspase-3的表达明显高于PBA组。RFBA组中Bcl-2的表达水平明显低于PBA组。
在28天时，RFBA可扩张动脉粥样硬化血管并增厚动脉粥样硬化斑块的纤维帽以促进斑块稳定性。还发现RFBA可激活凋亡因子和TGF-/Smad-2炎症信号通路。
