Kamada Hachiro, Matsui Yoshitaka
Institute of livestock and Grassland Science, NARO, Tsukuba, 305-0901, Japan.
Present address: Tohoku Agricultural Research Center, NARO, Shimokuriyagawa, Morioka, 020-0198, Japan.
Anim Biosci. 2023 Sep;36(9):1376-1383. doi: 10.5713/ab.22.0443. Epub 2023 Feb 26.
We aimed to determine the effects of 12-oxoeicosatetraenoic acid (12-KETE)-induced placenta release on the performance of mother cows (milk yield, ovarian function, and blood plasma biochemical properties).
Experimental treatments were as follows: i) natural delivery including natural placental release (control cows); ii) induced calf delivery with placental retention (RP cows); and iii) induced calf delivery and 12-KETE-induced placental release (KE cows). Delivery in pregnant KE cows was induced with dexamethasone and prostaglandin. These cows were injected with 12-KETE after calf discharge, resulting in the release of the fetal placenta. RP cows were not treated with 12-KETE after inducing delivery, resulting in placental retention.
The milk yield in RP cows during the first 50 days after delivery was significantly lower than that in control cows (p<0.05), whereas KE cows exhibited a similar milk yield to that of control cows. The postpartum plasma progesterone levels of control cows increased 14 days after delivery on average; however, its increase was delayed by 10 days in RP cows. Meanwhile, the 12-KETE treatment (KE cows) brought the timing of progesterone increase forward to the normal level (control cows). Among the 20 biochemical parameters examined, the total cholesterol levels in blood plasma 14 days after delivery were lower in RP cows than that in the other two treatment groups (control cows and KE cows) (p<0.05). In addition, the plasma level of haptoglobin tended to be low in cows that discharged their placentas shortly after delivery.
These findings indicate that 12-KETE treatment can alleviate the disorder caused by placental retention.
我们旨在确定12-氧代二十碳四烯酸（12-KETE）诱导的胎盘排出对母牛性能（产奶量、卵巢功能和血浆生化特性）的影响。
实验处理如下：i）自然分娩包括自然胎盘排出（对照母牛）；ii）诱导犊牛分娩但胎盘滞留（RP母牛）；iii）诱导犊牛分娩并12-KETE诱导胎盘排出（KE母牛）。对怀孕的KE母牛用地塞米松和前列腺素诱导分娩。这些母牛在犊牛产出后注射12-KETE，导致胎儿胎盘排出。诱导分娩后，RP母牛未用12-KETE处理，导致胎盘滞留。
RP母牛分娩后前50天的产奶量显著低于对照母牛（p<0.05），而KE母牛的产奶量与对照母牛相似。对照母牛产后血浆孕酮水平平均在分娩后14天升高；然而，RP母牛的升高延迟了10天。同时，12-KETE处理（KE母牛）使孕酮升高的时间提前至正常水平（对照母牛）。在检测的20项生化参数中，RP母牛分娩后14天血浆总胆固醇水平低于其他两个处理组（对照母牛和KE母牛）（p<0.05）。此外，产后不久排出胎盘的母牛血浆触珠蛋白水平往往较低。
这些发现表明，12-KETE处理可减轻胎盘滞留引起的紊乱。
