Department of Biomedical Education and Anatomy, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
School of Nursing, The Ohio State University, Columbus, OH 43210, USA.
Cleft Palate Craniofac J. 2024 Aug;61(8):1389-1397. doi: 10.1177/10556656231163400. Epub 2023 Mar 14.
Develop a model for the study of Electronic Nicotine Device (ENDS) exposure on craniofacial development.
Experimental preclinical design followed as pregnant murine dams were randomized and exposed to filtered air exposure, carrier exposure consisting of 50% volume of propylene glycol and vegetable glycine (ENDS Carrier) respectively, or carrier exposure with 20 mg/ml of nicotine added to the liquid vaporizer (ENDS carrier with nicotine).
Preclinical murine model exposure using the SciReq exposure system.
C57BL6 adult 8 week old female pregnant mice and exposed in utero litters.
Exposure to control filtered air, ENDS carrier or ENDS carrier with nicotine added throughout gestation at 1 puff/minute, 4 h/day, five days a week.
Cephalometric measures of post-natal day 15 pups born as exposed litters.
Data suggests alterations to several facial morphology parameters in the developing offspring, suggesting electronic nicotine device systems may alter facial growth if used during pregnancy.
Future research should concentrate on varied formulations and exposure regimens of ENDS to determine timing windows of exposures and ENDS formulations that may be harmful to craniofacial development.
建立电子尼古丁设备（ENDS）暴露对颅面发育影响的研究模型。
采用实验性临床前设计，将怀孕的雌性小鼠随机分为四组，分别暴露于过滤空气、含有 50%丙二醇和蔬菜甘油的载体（ENDS 载体）、或液体蒸发器中添加 20mg/ml 尼古丁的载体（添加尼古丁的 ENDS 载体）中。
使用 SciReq 暴露系统进行临床前的小鼠模型暴露。
C57BL6 成年 8 周龄雌性怀孕小鼠及其宫内胎鼠。
在整个孕期，以 1 口/分钟、4 小时/天、每周 5 天的频率，对控制过滤空气、ENDS 载体或添加尼古丁的 ENDS 载体进行暴露。
出生后 15 天的胎鼠的颅面测量指标。
数据表明，暴露于电子尼古丁设备系统的后代的几种面部形态参数发生了改变，这表明如果在怀孕期间使用电子尼古丁设备系统，可能会改变面部生长。
未来的研究应集中于不同配方和暴露方案的电子尼古丁设备，以确定对颅面发育有害的暴露时间窗口和电子尼古丁设备配方。
