Department of Otolaryngology Head and Neck Surgery, Tianjin First Center Hospital, Tianjin, PR China;
Department of Otolaryngology Head and Neck Surgery, Tianjin First Center Hospital, Tianjin, PR China.
Allergol Immunopathol (Madr). 2023 Mar 1;51(2):120-125. doi: 10.15586/aei.v51i2.728. eCollection 2023.
Long intergenic noncoding RNA 00632 (LINC00632) regulates nasal inflammation and CD4 T cell differentiation into T helper (Th) 2 cells in allergic rhinitis (AR). This study aimed to explore the relationship between LINC00632 and Th1/Th2 balance, and the clinical value of LINC00632 in AR patients.
In total, 120 AR patients, 20 non-atopic obstructive snoring patients as disease controls (DCs), and 20 healthy controls (HCs) were recruited. Their LINC00632 expressions in peripheral blood mononuclear cells were detected by RT-qPCR.
LINC00632 expression was declined in AR patients compared with DCs and HCs (both ˂ 0.001). Moreover, LINC00632 could distinguish AR patients from DCs with an area under curve (AUC) of 0.795 (95% confidence interval [CI]: 0.701-0.889), and from HCs with an AUC of 0.895 (95%CI: 0.831-0.960). LINC00632 was positively related to Th1 cells ( = 0.037) and Th1/Th2 axis ( ˂ 0.001) in AR patients. In addition, LINC00632 was inversely associated with Th2 cells ( ˂ 0.001) and interleukin (IL)-4 ( = 0.010) in AR patients. Besides, LINC00632 was negatively related to rhinorrhea score ( = 0.019), itching score ( = 0.008), sneezing score ( = 0.004), and total nasal symptom score (TNSS) ( ˂ 0.001), but no correlation between LINC00632 and congestion score was observed ( = 0.093). During treatment, LINC00632 was elevated, while TNSS score was reduced (both ˂ 0.001). Furthermore, LINC00632 increment was associated with the reduction of TNSS score during the therapy ( = 0.005).
LINC00632 relates to milder Th1/Th2 imbalance, attenuated nasal symptoms, and better response during 4-week therapy in AR patients.
长链非编码 RNA 00632（LINC00632）可调节变应性鼻炎（AR）中的鼻炎症和 CD4 T 细胞向辅助性 T 细胞（Th）2 细胞的分化。本研究旨在探讨 LINC00632 与 Th1/Th2 平衡的关系，以及 LINC00632 在 AR 患者中的临床价值。
共纳入 120 例 AR 患者、20 例非变应性阻塞性打鼾患者（疾病对照组，DCs）和 20 例健康对照者（HCs）。采用 RT-qPCR 检测外周血单个核细胞中 LINC00632 的表达。
与 DCs 和 HCs 相比，AR 患者的 LINC00632 表达降低（均 ˂ 0.001）。此外，LINC00632 可通过 AUC 为 0.795（95%CI：0.701-0.889）区分 AR 患者与 DCs，通过 AUC 为 0.895（95%CI：0.831-0.960）区分 AR 患者与 HCs。在 AR 患者中，LINC00632 与 Th1 细胞呈正相关（ = 0.037），与 Th1/Th2 轴呈正相关（ ˂ 0.001）。此外，LINC00632 与 AR 患者的 Th2 细胞（ ˂ 0.001）和白细胞介素（IL）-4（ = 0.010）呈负相关。此外，LINC00632 与流涕评分（ = 0.019）、瘙痒评分（ = 0.008）、打喷嚏评分（ = 0.004）和总鼻部症状评分（TNSS）（ ˂ 0.001）呈负相关，而与鼻塞评分无相关性（ = 0.093）。治疗期间，LINC00632 水平升高，而 TNSS 评分降低（均 ˂ 0.001）。此外，在治疗过程中，LINC00632 的增加与 TNSS 评分的降低呈正相关（ = 0.005）。
在 AR 患者中，LINC00632 与更轻微的 Th1/Th2 失衡、减轻的鼻部症状以及 4 周治疗期间更好的反应相关。
