Viana de Sousa Tábata, Carolina Jordão Cláudia, Augusto Abreu-Pereira César, Gorayb Pereira Ana Luiza, Barbugli Paula Aboud, Klein Marlise Inêz, Pavarina Ana Claudia
Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, Univ Estadual Paulista - UNESP, Araraquara, SP, Brazil.
Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas (UNICAMP), Piracicaba, Brazil.
Biofouling. 2023 Jan;39(1):94-109. doi: 10.1080/08927014.2023.2189011. Epub 2023 Mar 14.
The present study aimed to evaluate the effectiveness of hydrogen peroxide (HO) combined with antimicrobial photodynamic therapy (aPDT) on biofilms formed by strains which are either susceptible to or resistant to fluconazole. Biofilms were grown and treated with HO, followed by the application of Photodithazine® (P) and red light-emitting diode (LED) (L) either separately or combined ( = 12). After the treatment, biofilms were evaluated by estimating colony-forming unit ml, extracellular matrix components [water -soluble and -insoluble polysaccharides, proteins, extracellular DNA (eDNA)], biomass (total and insoluble dry-weight), and protein concentration. Biofilms formed by both strains presented a significant reduction in cell viability, biomass, extracellular matrix components (both types of polysaccharides, eDNA), and proteins (in the soluble and insoluble portion of biofilms) compared to the control. Microscopy images of the biofilms after treatments showed disarticulation of the matrix and scattered fungal cells. The application of HO can disturb the organization of the extracellular matrix, and its association with aPDT potentiated the effect of the treatment.
本研究旨在评估过氧化氢（HO）联合抗菌光动力疗法（aPDT）对氟康唑敏感或耐药菌株形成的生物膜的有效性。培养生物膜并用HO处理，然后分别或联合应用光二噻嗪（P）和红光发光二极管（LED）（每组n = 12）。处理后，通过估计菌落形成单位/毫升、细胞外基质成分[水溶性和水不溶性多糖、蛋白质、细胞外DNA（eDNA）]、生物量（总干重和不溶性干重）和蛋白质浓度来评估生物膜。与对照组相比，两种菌株形成的生物膜在细胞活力、生物量、细胞外基质成分（两种类型的多糖、eDNA）和蛋白质（生物膜的可溶性和不溶性部分）方面均有显著降低。处理后生物膜的显微镜图像显示基质解体和真菌细胞散在分布。HO的应用可扰乱细胞外基质的组织结构，其与aPDT联合使用可增强治疗效果。
