United States Department of Agriculture- Agricultural Research Service, U.S. National Poultry Research Center, Poultry Microbiological Safety and Processing Research Unit, 950 College Station Road, Athens, GA 30605, USA.
United States Department of Agriculture- Agricultural Research Service, U.S. National Poultry Research Center, Poultry Microbiological Safety and Processing Research Unit, 950 College Station Road, Athens, GA 30605, USA.
J Food Prot. 2023 Feb;86(2):100033. doi: 10.1016/j.jfp.2022.100033. Epub 2022 Dec 23.
Salmonella enterica is a major cause of human foodborne illness and is often attributed to poultry food sources. S. enterica serovar Infantis, specifically those carrying the pESI plasmid, has become a frequently isolated serotype from poultry meat samples at processing and has caused numerous recent human infections. In 2016, the USDA-Food Safety and Inspection Service changed the official sampling method for raw poultry products from BPW to using neutralizing BPW (nBPW) as the rinsing agent in order to prevent residual antimicrobial effects from acidifying and oxidizing processing aids. This change was contemporaneous to the emergence of pESI-positive ser. Infantis as a prevalent serovar in poultry, prompting some to question if nBPW could be selecting for this prevalent serovar. We performed two experiments: a comparison of ser. Infantis growth in BPW versus nBPW, and a simulation of regulatory sampling methods. We found that when inoculated into both broths, ser. Infantis initially grows slightly slower in nBPW than in BPW but little difference was seen in abundance after 6 h of growth. Additionally, the use of nBPW to simulate poultry rinse sample and overnight cold shipping to a regulatory lab did not affect the survival or subsequent growth of ser. Infantis in BPW. We concluded that the change in USDA-FSIS methodology to include nBPW in sampling procedures has likely not affected the emergence of S. ser. Infantis as a prevalent serovar in chicken and turkey meat product samples.
肠炎沙门氏菌是导致人类食源性疾病的主要原因，通常与禽类食物来源有关。肠炎沙门氏菌血清型婴儿型，特别是那些携带 pESI 质粒的血清型，已成为加工过程中从禽肉样本中经常分离到的血清型，并导致了最近许多人类感染。2016 年，美国农业部-食品安全检验局将生禽产品的官方采样方法从 BPW 改为使用中和 BPW（nBPW）作为冲洗剂，以防止酸化和氧化加工助剂的残留抗菌效果。这一变化与 pESI 阳性血清型 Infantis 作为禽类中流行血清型的出现同时发生，这促使一些人质疑 nBPW 是否可以选择这种流行的血清型。我们进行了两项实验：比较血清型 Infantis 在 BPW 与 nBPW 中的生长情况，以及模拟监管采样方法。我们发现，当接种到两种肉汤中时，血清型 Infantis 最初在 nBPW 中的生长速度比在 BPW 中略慢，但在 6 小时的生长后，丰度差异很小。此外，使用 nBPW 模拟禽类冲洗样本并隔夜冷藏运输到监管实验室，并不影响血清型 Infantis 在 BPW 中的存活或随后的生长。我们得出结论，美国农业部-食品安全检验局方法的改变，即在采样程序中包含 nBPW，可能没有影响 S.血清型 Infantis 作为鸡和火鸡肉产品样本中流行血清型的出现。
