促进成肌细胞增殖的牛胶原蛋白水解产物中肽的鉴定与表征

Identification and Characterization of Peptides from Bovine Collagen Hydrolysates that Promote Myogenic Cell Proliferation.

作者信息

Li Hanfeng, Chen Xin, Zuo Zhijie, Wang Jianing, Guo Yanchuan

机构信息

Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

School of Chemical Sciences, University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China.

出版信息

J Agric Food Chem. 2023 Mar 29;71(12):4876-4889. doi: 10.1021/acs.jafc.2c08929. Epub 2023 Mar 14.

DOI:10.1021/acs.jafc.2c08929

PMID:36917229

Abstract

In this study, bovine collagen hydrolysate was purified via a series of chromatograms, and the peptides with the highest activity for promoting myoblast proliferation were identified by LC-MS-MS. It was demonstrated that the peptide GDAGPGPAGPAGPGPIG (hydroxylation) could promote C2C12 proliferation (+18.5% ± 0.04, < 0.05). The certain peptide was capable of regulating the myogenic cell cycle and inhibiting myogenic cell apoptosis. By combining molecular docking, quantitative real-time PCR, and metabonomics, we suggested that the peptide GDAGPGPAGPAGPGPIG (hydroxylation) might bind to FGFR1 and affect the expression of genes downstream of FGFR1 and influence protein synthesis to promote myoblast proliferation. The above results showed that the peptides isolated in this study have the potential to alleviate sarcopenia in the elderly.

摘要

在本研究中，通过一系列色谱法对牛胶原蛋白水解产物进行了纯化，并通过液相色谱-串联质谱法鉴定出对促进成肌细胞增殖具有最高活性的肽。结果表明，肽GDAGPGPAGPAGPGPIG（羟基化）可促进C2C12增殖（增加18.5%±0.04，P<0.05）。该特定肽能够调节成肌细胞周期并抑制成肌细胞凋亡。通过结合分子对接、定量实时聚合酶链反应和代谢组学，我们认为肽GDAGPGPAGPAGPGPIG（羟基化）可能与成纤维细胞生长因子受体1（FGFR1）结合，并影响FGFR1下游基因的表达，进而影响蛋白质合成以促进成肌细胞增殖。上述结果表明，本研究中分离出的肽具有缓解老年人肌肉减少症的潜力。