Department of Materials Science and Nanotechnology, Graduate School of Natural and Applied Sciences, Konya Food and Agriculture University, 42080 Konya, Türkiye.
Department of Bioengineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, 42080 Konya, Türkiye.
ACS Appl Bio Mater. 2023 Apr 17;6(4):1604-1610. doi: 10.1021/acsabm.3c00021. Epub 2023 Mar 14.
Increased bacterial resistance against extensively used common disinfectants has begun to emerge. The discovery of disinfectants substituting the current commercially available ones is strongly needed. For this purpose, a dicationic BODIPY-based fluorescent amphiphile has been synthesized by specific molecular design. This quaternized BODIPY behaves as a broad-spectrum disinfectant against both Gram-positive and Gram-negative bacteria strains. It exhibits potent antimicrobial activity against tested microorganisms when compared with structurally similar disinfectant benzalkonium chloride (BAC). Moreover, it shows antibiofilm activity against with a minimum biofilm eradication concentration as low as 16 μg/mL. The interaction of this compound with the bacterial cell and genomic DNA was further evaluated by fluorescence spectroscopy and microscopy to follow cell internationalization and to clarify the mechanism of antibacterial action.
对抗生素广泛使用的常见消毒剂的细菌耐药性已开始出现。因此，强烈需要发现可替代当前市售消毒剂的消毒剂。为此，通过特定的分子设计合成了一种双阳离子基于 BODIPY 的荧光两亲分子。这种季铵化 BODIPY 可作为一种广谱消毒剂，对革兰氏阳性和革兰氏阴性细菌菌株均有效。与结构相似的消毒剂苯扎氯铵（BAC）相比，它对测试的微生物表现出很强的抗菌活性。此外，它对生物膜表现出抗生物膜活性，最低生物膜清除浓度低至 16 μg/mL。通过荧光光谱和显微镜进一步评估了该化合物与细菌细胞和基因组 DNA 的相互作用，以跟踪细胞内化并阐明抗菌作用机制。
