College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Inner Mongolia Autonomous Region Water Conservancy Development Center, Hohhot, Inner Mongolia, China.
PeerJ. 2023 Mar 9;11:e14978. doi: 10.7717/peerj.14978. eCollection 2023.
Fine roots are vital to a plant's ability to absorb water and nutrients. Stumping is a practice that may encourage fine root growth and the rapid recovery of decaying plants. However, the effect of stumping on the fine roots and physiological indices is still unknown. The differential indices between stumped forests and non-stumped forests must also be defined.
We recorded the changes in the fine roots of structure one year after stumping. Using single factor analysis of variance and general linear models we comprehensively analyzed the number of root tips and the plant's growth and physiological indices in response to stumping. Partial least squares discriminant analysis (PLS-DA) was used to compare fine root growth and physiological indices with and without stumping in order to identify the differential indices.
The proportion of root tips in the vertical layers at 30-40 cm and 40-50 cm and in the horizontal layers at 60-80 cm and 80-100 cm, increased after stumping by 1.85%, 2.60%, 1.96% and 4.32%, respectively. In the 0-50 cm soil layer, the fine root dry weight rose by 27.6% compared with the control, which was not significant. However, other indices were significantly different from the control. The proportions in the growth indices in the 30-40 cm and 40-50 cm layers increased after stumping. Stumping had a significant, negative effect on proline and malondialdehyde content, which dropped by 40.95% and 55.32%, respectively, indicating that the harms caused by these two chemicals was alleviated. Stumping had a significant positive effect on root activity and soluble sugar contents, which increased by 68.58% and 36.87%, respectively, and improved the growing ability of fine roots. PLS-DA revealed that malondialdehyde, soluble sugars, root density, and the number of root tips ranked from having the least to greatest effect on the classification of stumping and no-stumping.
The process of stumping may promote fine roots growth in , and is favorable for their longitudinal development. The fine root growing indices of responded positively to this process. Stumping promotes root activity and the creation of soluble sugar to maintain the growth and development of fine roots. It also inhibits the negative effects of proline and malondialdehyde on fine roots. Our study showed that the differential physiological indices were more important for classification than the differential growing indices.
细根对于植物吸收水分和养分的能力至关重要。砍桩是一种可以促进细根生长和加速腐烂植物恢复的做法。然而，砍桩对细根和生理指标的影响尚不清楚。还必须定义砍桩林与未砍桩林之间的差异指标。
我们记录了砍桩一年后结构层中细根的变化。使用单因素方差分析和一般线性模型，我们综合分析了根尖端数量以及植物对砍桩的生长和生理指标的响应。偏最小二乘判别分析（PLS-DA）用于比较砍桩和不砍桩对细根生长和生理指标的影响，以确定差异指标。
砍桩后，30-40cm 和 40-50cm 垂直层以及 60-80cm 和 80-100cm 水平层的根尖端比例分别增加了 1.85%、2.60%、1.96%和 4.32%。在 0-50cm 土壤层中，与对照相比，细根干重增加了 27.6%，但差异不显著。然而，其他指标与对照有显著差异。在 30-40cm 和 40-50cm 层的生长指标中，砍桩后比例增加。砍桩对脯氨酸和丙二醛含量有显著的负效应，分别下降了 40.95%和 55.32%，表明这两种化学物质造成的危害得到了缓解。砍桩对根活力和可溶性糖含量有显著的正效应，分别增加了 68.58%和 36.87%，提高了细根的生长能力。PLS-DA 表明，丙二醛、可溶性糖、根密度和根尖端数量对砍桩和不砍桩的分类影响最小到最大。
砍桩过程可能促进细根生长，有利于其纵向发育。细根生长指标对这一过程呈正响应。砍桩促进根活力和可溶性糖的产生，以维持细根的生长和发育。它还抑制了脯氨酸和丙二醛对细根的负面影响。我们的研究表明，与生长指标相比，生理差异指标对分类更为重要。
