miR-127 表达下调通过上调 SPP1 的表达促进乳腺癌的发生。

Decreased miR-127 promotes the occurrence of breast cancer via increasing the expression of SPP1.

机构信息

Center for Clinical Laboratory Diagnosis and Research, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.

Baise Key Laboratory of Clinical Molecular Diagnosis, Research and Development for High Incidence Diseases, China.

出版信息

Adv Clin Exp Med. 2023 Oct;32(10):1113-1123. doi: 10.17219/acem/161161.

DOI:10.17219/acem/161161

PMID:36920269

Abstract

BACKGROUND

The expression of miR-127 has been reported to be decreased in the breast tissue of patients with breast cancer (BRC). However, the mechanism of miR-127 involvement in the pathogenesis of BRC is still unclear and requires urgent clarification.

OBJECTIVES

To explore the role of miR-127 in the pathogenesis of BRC.

MATERIAL AND METHODS

In this study, we measured the expression of miR-127 in blood samples of 60 BRC patients and 60 controls, investigated the influence of miR-127 on the viability and apoptosis of MCF-7 and MDA-231 cells, identified a miR-127 target gene, and determined the expression level of the target gene in the blood samples of BRC patients and controls.

RESULTS

We found that miR-127 expression was significantly decreased in the plasma of BRC patients compared to controls. Additionally, the upregulation of miR-127 in MCF-7 and MDA-231 cells inhibited their proliferation and promoted their apoptosis. Conversely, the downregulation of miR-127 promoted cell proliferation and inhibited their apoptosis. The SPP1 was successively predicted and validated as a target gene of miR-127. Finally, the expression level of SPP1 was significantly increased in the plasma of BRC patients compared to controls.

CONCLUSIONS

Our study demonstrated that decreased miR-127 may promote BRC cell proliferation, inhibit apoptosis and promote the occurrence of BRC through increasing the SPP1 expression level.

摘要

背景

已有研究报道，miR-127 在乳腺癌（BRC）患者的乳腺组织中表达降低。然而，miR-127 参与 BRC 发病机制的具体机制尚不清楚，需要进一步阐明。

目的

探索 miR-127 在 BRC 发病机制中的作用。

材料与方法

本研究测量了 60 例 BRC 患者和 60 例对照者的血液样本中 miR-127 的表达情况，研究了 miR-127 对 MCF-7 和 MDA-231 细胞活力和凋亡的影响，鉴定了 miR-127 的靶基因，并检测了 BRC 患者和对照者血液样本中靶基因的表达水平。

结果

我们发现，与对照者相比，BRC 患者的血浆中 miR-127 表达显著降低。此外，上调 MCF-7 和 MDA-231 细胞中的 miR-127 抑制了它们的增殖并促进了它们的凋亡。相反，下调 miR-127 则促进了细胞增殖并抑制了细胞凋亡。SPP1 被成功预测和验证为 miR-127 的靶基因。最后，与对照者相比，BRC 患者的血浆中 SPP1 的表达水平显著升高。