Hsu Li-Chuan, Li Chia-Jung, Lin Li-Te, Pan Li-Fei, Wen Zhi-Hong, Sheu Jim Jinn-Chyuan, Tsui Kuan-Hao
Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Department of Obstetrics and Gynaecology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.
J Pers Med. 2024 Jan 9;14(1):78. doi: 10.3390/jpm14010078.
Secreted phosphoprotein 1 (SPP1), also known as osteopontin (OPN), is located on chromosome 4q22.1. This multifunctional secreted acidic glycoprotein is expressed intracellularly and extracellularly in various tissues, where it interacts with regulatory proteins and pro-inflammatory immune chemokines, contributing to the pathogenesis of multiple diseases. Nevertheless, the intricate genetic connections between SPP1 and ovarian aging remain largely unexplored. This study aims to bridge this knowledge gap by delving into ovarian aging and its associations with SPP1 using multi-omics data analysis. Our findings indicate that SPP1 is a potential gene related to ovarian aging. To comprehend the role of SPP1, we conducted spatial transcriptomic analyses on young and aged female mouse ovaries, revealing a significant decline in SPP1 expression in the aging group compared to the young group. Similarly, a significantly low level of SPP1 was found in the 73-year-old sample. Additionally, in-depth single-cell RNA-sequencing analysis identified associations between SPP1 and ITGAV, ITGB1, CD44, MMP3, and FN1. Notably, co-expression analysis highlighted a strong correlation between SPP1 and ITGB1. In summary, this study pioneers the identification of SPP1 as a gene implicated in ovarian aging. Further research into the role of SPP1 has the potential to advance precision medicine and improve treatment strategies for ovarian aging-related conditions.
分泌型磷蛋白1（SPP1），也称为骨桥蛋白（OPN），位于4号染色体q22.1区域。这种多功能分泌酸性糖蛋白在多种组织的细胞内和细胞外均有表达，在这些组织中它与调节蛋白和促炎免疫趋化因子相互作用，参与多种疾病的发病机制。然而，SPP1与卵巢衰老之间复杂的遗传联系在很大程度上仍未被探索。本研究旨在通过使用多组学数据分析深入研究卵巢衰老及其与SPP1的关联，以填补这一知识空白。我们的研究结果表明，SPP1是一个与卵巢衰老相关的潜在基因。为了理解SPP1的作用，我们对年轻和老年雌性小鼠卵巢进行了空间转录组分析，结果显示与年轻组相比，衰老组中SPP1表达显著下降。同样，在73岁的样本中也发现SPP1水平显著较低。此外，深入的单细胞RNA测序分析确定了SPP1与整合素αV（ITGAV）、整合素β1（ITGB1）、CD44、基质金属蛋白酶3（MMP3）和纤连蛋白1（FN1）之间的关联。值得注意 的是，共表达分析突出了SPP1与ITGB1之间的强相关性。总之，本研究首次确定SPP1为与卵巢衰老相关的基因。对SPP1作用的进一步研究有可能推动精准医学发展，并改善卵巢衰老相关病症的治疗策略。
