Liu Chen-Xu, Bai Xiao-Hui
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Huan Jing Ke Xue. 2023 Mar 8;44(3):1537-1541. doi: 10.13227/j.hjkx.202204208.
To study the distribution characteristics and horizontal transfer potential of antibiotic resistance genes in biofilms of water supply pipes made of different materials, the biofilms of 304 and 316 L stainless steel pipes and PPR and PE plastic pipes were analyzed using metagenomic sequencing. The results showed that a total of 146 antibiotic resistance genes belonging to 17 classes were detected in the biofilms. Multidrug resistance genes had the highest abundance and varied a lot in different biofilms, which was the main reason for the differences in antibiotic resistance genes in different biofilms. The total abundance of mobile genetic elements was high in different biofilms, but overall coexistence with antibiotic resistance genes was low, with integrase genes showing a closer relationship with antibiotic resistance genes and possibly playing an important role in the horizontal transfer of antibiotic resistance genes. Different antibiotic resistance genes possessed different genera of potential hosts; highly abundant hosts such as and were the basis for the presence of a high abundance of multidrug resistance genes. Microbial attachment in different pipe walls was selective, and the primary influence of antibiotic resistance in biofilms was bacterial community composition, followed by the horizontal transfer of genes mediated by mobile genetic elements, community composition, and mobile genetic elements together shaping the distribution characteristics of antibiotic resistance genes.
为研究不同材质给水管壁生物膜中抗生素抗性基因的分布特征及水平转移潜力，采用宏基因组测序技术对304和316L不锈钢管以及PPR和PE塑料管的生物膜进行分析。结果表明，在生物膜中共检测到17类146种抗生素抗性基因。多药抗性基因丰度最高，且在不同生物膜中差异较大，这是不同生物膜中抗生素抗性基因存在差异的主要原因。不同生物膜中可移动遗传元件的总丰度较高，但与抗生素抗性基因的总体共存率较低，其中整合酶基因与抗生素抗性基因关系更为密切，可能在抗生素抗性基因的水平转移中发挥重要作用。不同抗生素抗性基因具有不同属的潜在宿主；如 和 等丰度较高的宿主是多药抗性基因高丰度存在的基础。不同管壁上的微生物附着具有选择性，生物膜中抗生素抗性的主要影响因素是细菌群落组成，其次是可移动遗传元件介导的基因水平转移，群落组成和可移动遗传元件共同塑造了抗生素抗性基因的分布特征。
