Li Xiao, Li Jie, Zhou Pan, Li Danni, Wang Mingxin, Tong Qianqian, Chen Jian, Zuo Changjing, Zhang Lan, Li Rou
Shanghai Institute of Applied Physics, Chinese Academy of Sciences Shanghai 201800, China.
University of Chinese Academy of Sciences Beijing 100049, China.
Am J Nucl Med Mol Imaging. 2023 Feb 15;13(1):43-50. eCollection 2023.
Molecular imaging can dynamically and quantitatively record the biochemical changes in a systemic view. In this research, SARS-CoV-2 pseudovirus was intramuscularly injected to simulate the vaccination with inactivated virus. New Zealand white rabbits were evaluated with F-FDG PET for inflammation and Ga-cyc-DX600 PET for ACE2 fluctuation, which were performed before and at 3, 7 and 14 days post injection (d P.I.); furthermore, one rabbit was vaccinated with two cycles with interval of 14 days for a longer period evaluation. Different with the vaccination-induced inflammatory response that was random and individual, ACE2 regulation was systemic and organ-specific: the liver and spleen were of a moderate decrease post injection but rebound at 14 d P.I., while there were a downward trend in heart, testis and bone marrow; besides, similar pattern of ACE2 regulation were recorded after the second injection with a relatively greater volatility. In conclusion, ACE2 PET gave a more comprehensive view on host response post vaccination, hold substantial promise in continuous monitoring of coronavirus vaccine administration and effectiveness.
分子成像能够从系统层面动态且定量地记录生物化学变化。在本研究中，通过肌肉注射严重急性呼吸综合征冠状病毒2（SARS-CoV-2）假病毒来模拟灭活病毒疫苗接种。对新西兰白兔进行氟代脱氧葡萄糖（F-FDG）正电子发射断层显像（PET）以评估炎症情况，进行镓标记环孢素-DX600（Ga-cyc-DX600）PET以评估血管紧张素转换酶2（ACE2）的波动情况，分别在注射前以及注射后3天、7天和14天进行检查；此外，为进行更长时间的评估，对一只兔子间隔14天进行两个周期的疫苗接种。与疫苗接种引发的随机且个体性的炎症反应不同，ACE2的调节是全身性且具有器官特异性的：肝脏和脾脏在注射后出现中度下降，但在注射后14天出现反弹，而心脏、睾丸和骨髓则呈下降趋势；此外，第二次注射后记录到类似的ACE2调节模式，且波动相对更大。总之，ACE2 PET能更全面地观察疫苗接种后的宿主反应，在持续监测冠状病毒疫苗接种及效果方面具有巨大潜力。
