Department of Cardiovascular Ultrasound, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
Clin Exp Hypertens. 2023 Dec 31;45(1):2190503. doi: 10.1080/10641963.2023.2190503.
Pulmonary arterial hypertension (PAH) means high blood pressure in the lungs. We aimed to observe the right ventricular size, wall thickness and characteristic functional changes and their associations with PAH in an established model of beagle dogs, and to explore convenient, reliable and sensitive ultrasound indicators for assessing right ventricular remodeling.
Twenty healthy beagle dogs (8-10 kg) were randomly divided into control group (N-dimethylformamide, = 10) and dehydromonocrotaline (DHMCT) group (DHMCT, = 10). N-dimethylformamide or DHMCT was injected through a catheter into the right atrium, and then right heart catheterization, routine echocardiography and two-dimensional speckle tracking imaging (2D-STI) were performed before modeling (0 weeks) and 8, 14 weeks after modeling. Hemodynamic parameters and right ventricular function-related ultrasound data were acquired. At the end of the experiment, the animals were killed and the lung tissues were taken for HE staining. Left and right ventricular walls were separated and weighed respectively, and right ventricular hypertrophy index (RVHI) was measured. The associations of the routine ultrasound data and 2D-STI data at each time point with hemodynamic parameters and RVHI were analyzed.
At 0, 8 and 14 weeks, gradual decreases in the right ventricular global longitudinal strain (RVLS) were found in DHMCT group. RVH occurred in DHMCT group, and DHMCT group had a significantly higher RVHI than that of control group (49.83 ± 4.83% vs. 39.80 ± 1.40%, < .001) and larger pulmonary artery media thickness. RVLS had significant positive correlations with RVSP ( = 0.74, < .001), mRVP ( = 0.72, < .001), PASP ( = 0.75, < .001), mPAP ( = 0.72, < .001) and PVR ( = 0.68, < .001). There was a significant positive correlation between RVLS and RVHI ( = 0.74, < .001).
The right ventricular function in PAH can be effectively assessed by echocardiography, and RVLS measured by 2D-STI sensitively reflects right ventricular remodeling following PAH.
肺动脉高压（PAH）是指肺部血压升高。本研究旨在观察建立的比格犬模型中右心室大小、壁厚度和特征性功能变化及其与 PAH 的关系，并探讨评估右心室重构的方便、可靠和敏感的超声指标。
20 只健康比格犬（8-10kg）随机分为对照组（N-二甲基甲酰胺，N=10）和脱水单环酸（DHMCT）组（DHMCT，N=10）。N-二甲基甲酰胺或 DHMCT 通过导管注入右心房，然后在建模前（0 周）和建模后 8、14 周进行右心导管检查、常规超声心动图和二维斑点追踪成像（2D-STI）。获取血流动力学参数和与右心室功能相关的超声数据。实验结束时处死动物，取肺组织行 HE 染色。分别分离左、右心室壁并称重，测量右心室肥厚指数（RVHI）。分析各时间点常规超声数据和 2D-STI 数据与血流动力学参数和 RVHI 的相关性。
在 0、8 和 14 周时，DHMCT 组右心室整体纵向应变（RVLS）逐渐降低。DHMCT 组发生右心室肥大，与对照组相比，DHMCT 组的 RVHI 明显更高（49.83±4.83%比 39.80±1.40%， < .001），肺动脉中层厚度更大。RVLS 与 RVSP（ = 0.74， < .001）、mRVP（ = 0.72， < .001）、PASP（ = 0.75， < .001）、mPAP（ = 0.72， < .001）和 PVR（ = 0.68， < .001）呈显著正相关。RVLS 与 RVHI 呈显著正相关（ = 0.74， < .001）。
超声心动图可有效评估 PAH 右心室功能，2D-STI 测量的 RVLS 敏感反映 PAH 后右心室重构。
