Wang Ting, Ji Bing, Cheng Zehua, Chen Ling, Luo Mai, Wei Jinchao, Wang Yuefei, Zou Liang, Liang Yuanzhe, Zhou Bingpu, Li Peng
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
Institute of Applied Physics and Materials Engineering, University of Macau, Macau, China; School of Physics and Electronics, Hunan Normal University, Changsha, 410081, China.
Biosens Bioelectron. 2023 May 15;228:115191. doi: 10.1016/j.bios.2023.115191. Epub 2023 Mar 4.
Researchers have struggled to develop highly reliable and sensitive surface-enhanced Raman scattering (SERS) substrates for detecting compounds in complicated systems. In this work, a strategy by constructing Au cores with incompletely wrapped Prussian blue (PB) for highly reliable and sensitive SERS substrate is proposed. The wrapped PB layers can provide the internal standard (IS) to calibrate the SERS signal floatation, whereas the exposed surface of Au cores offers the enhancement effect. The balance between the signal self-calibration and enhancement (hence the trade-off between SERS reliability and sensitivity) is obtained by the approximate semi-wrapping configuration of PB layers on Au cores (i.e., SW-Au@PB). The proposed SW-Au@PB nanoparticles (NPs) exhibit the similar enhancement factor as the pristine Au NPs and contribute to the ultralow RSD (8.55%) of calibrated SERS signals using R6G as probe molecules. The simultaneously realized reliability and sensitivity of SW-Au@PB NPs also enables the detection of hazardous pesticide residues such as paraquat and thiram in herbal plants, with the average detection accuracy up to 92%. Overall, this work mainly provides a controllable synthetic strategy for incompletely wrapped NPs, and most importantly, explores the potential with a proof-of-concept practical application in accurate and sensitive Raman detection of hazardous substances with varying solubility.
研究人员一直致力于开发高度可靠且灵敏的表面增强拉曼散射（SERS）基底，用于检测复杂体系中的化合物。在这项工作中，提出了一种通过构建不完全包裹普鲁士蓝（PB）的金核来制备高度可靠且灵敏的SERS基底的策略。包裹的PB层可提供内标（IS）以校准SERS信号波动，而金核的暴露表面则提供增强效果。通过PB层在金核上近似半包裹的结构（即SW-Au@PB）实现了信号自校准与增强之间的平衡（从而实现了SERS可靠性与灵敏度之间的权衡）。所提出的SW-Au@PB纳米颗粒（NPs）表现出与原始金纳米颗粒相似的增强因子，并有助于使用R6G作为探针分子时校准SERS信号的超低相对标准偏差（RSD，8.55%）。SW-Au@PB NPs同时实现的可靠性和灵敏度还能够检测草药植物中的百草枯和福美双等有害农药残留，平均检测准确率高达92%。总体而言，这项工作主要提供了一种制备不完全包裹纳米颗粒的可控合成策略，最重要的是，通过一个概念验证的实际应用探索了在准确灵敏地拉曼检测具有不同溶解度的有害物质方面的潜力。
