基于基因表达综合数据库分析幼鼠脑缺血相关差异表达基因
Analysis of differentially expressed genes related to cerebral ischaemia in young rats based on the Gene Expression Omnibus database.
作者信息
Xia Yu, Liu Han, Zhu Rui
机构信息
Department of Neurology, The Third People's Hospital of Hefei (The Third Clinical College of Anhui Medical University), Hefei 230022, Anhui Province, China.
Department of Neurology, The First Affiliated Hospital of Anhui Medical University (Anhui Public Health Clinical Center), Hefei 230022, Anhui Province, China.
出版信息
World J Clin Cases. 2023 Mar 6;11(7):1467-1476. doi: 10.12998/wjcc.v11.i7.1467.
BACKGROUND
The incidence rate of cerebral infarction in young people is increasing day by day, the age of onset tends to be younger, and its internal pathogenesis and mechanism are very complicated, which leads to greater difficulties in treatment. Therefore, it is essential to analyze the key pathway that affects the onset of cerebral infarction in young people from the perspective of genetics.
AIM
To compare the differentially expressed genes in the brain tissue of young and aged rats with middle cerebral artery occlusion and to analyse their effect on the key signalling pathway involved in the development of cerebral ischaemia in young rats.
METHODS
The Gene Expression Omnibus 2R online analysis tool was used to analyse the differentially expressed genes in the GSE166162 dataset regarding the development of cerebral ischaemia in young and aged groups of rats. DAVID 6.8 software was further used to filter the differentially expressed genes. These genes were subjected to Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to determine the key gene pathway that affects the occurrence of cerebral ischaemia in young rats.
RESULTS
Thirty-five differentially expressed genes (such as , and ) were obtained; 73 GO enrichment analysis pathways are mainly involved in biological processes such as drug response, amino acid stimulation response, blood vessel development, various signalling pathways, and enzyme regulation. They are involved in molecular functions such as drug binding, protein binding, dopamine binding, metal ion binding, and dopamine neurotransmitter receptor activity. KEGG pathway enrichment analysis showed a significantly enriched pathway: The cyclic adenosine monophosphate (c-AMP) signalling pathway.
CONCLUSION
The c-AMP signalling pathway might be the key pathway in the intervention of cerebral infarction in young people.
背景
青年人脑梗死发病率日益增高，发病年龄趋于年轻化，其内在发病机制非常复杂，导致治疗难度加大。因此，从遗传学角度分析影响青年人脑梗死发病的关键通路至关重要。
目的
比较大脑中动脉闭塞的青年和老年大鼠脑组织中差异表达基因，并分析其对青年大鼠脑缺血发生过程中关键信号通路的影响。
方法
使用基因表达综合数据库2R在线分析工具分析GSE166162数据集中青年和老年大鼠脑缺血发生相关的差异表达基因。进一步使用DAVID 6.8软件筛选差异表达基因。对这些基因进行基因本体（GO）功能分析和京都基因与基因组百科全书（KEGG）通路富集分析，以确定影响青年大鼠脑缺血发生的关键基因通路。
结果
获得35个差异表达基因（如 、 和 ）；73条GO富集分析通路主要涉及药物反应、氨基酸刺激反应、血管发育、各种信号通路和酶调节等生物学过程。它们涉及药物结合、蛋白质结合、多巴胺结合、金属离子结合和多巴胺神经递质受体活性等分子功能。KEGG通路富集分析显示一条显著富集的通路：环磷酸腺苷（c-AMP）信号通路。
结论
c-AMP信号通路可能是青年人脑梗死干预的关键通路。
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