• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状RNA-0076906和环状RNA-0134944的失调与中国汉族绝经后女性骨质疏松症及骨质疏松性骨折易感性相关。

Dysregulation of circRNA-0076906 and circRNA-0134944 is Correlated with Susceptibility to Osteoporosis and Osteoporotic Fracture in Postmenopausal Females from the Chinese Han Population.

作者信息

Yang Weijie, Zhang Wei, Li Fengqian, Xu Ning, Sun Ping

机构信息

Department of Orthopedics, Shanghai Eighth People's Hospital, Shanghai, 200235, People's Republic of China.

出版信息

Pharmgenomics Pers Med. 2023 Mar 10;16:183-194. doi: 10.2147/PGPM.S394757. eCollection 2023.

DOI:10.2147/PGPM.S394757
PMID:36926413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10013579/
Abstract

INTRODUCTION

Many circRNAs, such as circRNA-0076906 and circRNA-0134944, have been reported to participate in the pathogenesis of osteoporosis via sponging miRNAs in postmenopausal female patients. In this study, we aimed to study potential signaling pathways underlying the role of certain circRNAs, miRNAs and their target genes in the pathogenesis of osteoporotic fracture in postmenopausal females.

METHODS

Quantitative real-time PCR was performed to analyze the expression of circRNAs, miRNAs and their targets genes. Luciferase assays were carried out to explore the regulatory relationship between circ_0076906/miR-548i/OGN and circ_0134944/miR-630/TLR4.

RESULTS

Osteoporosis and fracture were positively correlated to the expression of circ_0134944, miR-548i and TLR4, but negatively correlated to the expression of circ_0076906, miR-630 and OGN in the peripheral blood and bone tissue samples of postmenopausal women. Luciferase activities of wild-type circ_0076906 and OGN were inhibited by miR-548i, and the luciferase activities of wild-type circ_0134944 and TLR4 were suppressed by miR-630 in MG-63 and U-2 OS cells. Inhibition of circ_0076906 expression in MG-63 and U-2 OS cells activated the expression of miR-548i and inhibited the expression of OGN. Moreover, the overexpression of circ_0134944 in MG-63 and U-2 OS cells suppressed the expression of miR-630 and enhanced the expression of TLR4.

CONCLUSION

This study implied that the dysregulation of circRNA-0076906 and circRNA-0134944 modulated their specific signaling and thus contributed to the severity of osteoporosis, increasing the risk of osteoporotic fracture.

摘要

引言

许多环状RNA，如circRNA - 0076906和circRNA - 0134944，已被报道在绝经后女性患者中通过吸附微小RNA参与骨质疏松症的发病机制。在本研究中，我们旨在研究某些环状RNA、微小RNA及其靶基因在绝经后女性骨质疏松性骨折发病机制中潜在的信号通路。

方法

采用定量实时PCR分析环状RNA、微小RNA及其靶基因的表达。进行荧光素酶报告基因检测以探究circ_0076906/miR - 548i/OGN和circ_0134944/miR - 630/TLR4之间的调控关系。

结果

骨质疏松症和骨折与绝经后女性外周血和骨组织样本中circ_0134944、miR - 548i和TLR4的表达呈正相关，但与circ_0076906、miR - 630和OGN的表达呈负相关。在MG - 63和U - 2 OS细胞中，miR - 548i抑制野生型circ_0076906和OGN的荧光素酶活性，miR - 630抑制野生型circ_0134944和TLR4的荧光素酶活性。在MG - 63和U - 2 OS细胞中抑制circ_0076906的表达可激活miR - 548i的表达并抑制OGN的表达。此外，在MG - 63和U - 2 OS细胞中过表达circ_0134944可抑制miR - 630的表达并增强TLR4的表达。

结论

本研究表明，circRNA - 0076906和circRNA - 0134944的失调调节了它们的特定信号通路，从而导致骨质疏松症的严重程度增加，增加了骨质疏松性骨折的风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/0a8eac963750/PGPM-16-183-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/7b7fe32976ba/PGPM-16-183-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/b84a8e8787e6/PGPM-16-183-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/4d4cd97fa57b/PGPM-16-183-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/b1e1e99f6f22/PGPM-16-183-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/210c86b79dc9/PGPM-16-183-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/90ae7e836071/PGPM-16-183-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/0a8eac963750/PGPM-16-183-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/7b7fe32976ba/PGPM-16-183-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/b84a8e8787e6/PGPM-16-183-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/4d4cd97fa57b/PGPM-16-183-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/b1e1e99f6f22/PGPM-16-183-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/210c86b79dc9/PGPM-16-183-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/90ae7e836071/PGPM-16-183-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8d6/10013579/0a8eac963750/PGPM-16-183-g0007.jpg

相似文献

1
Dysregulation of circRNA-0076906 and circRNA-0134944 is Correlated with Susceptibility to Osteoporosis and Osteoporotic Fracture in Postmenopausal Females from the Chinese Han Population.环状RNA-0076906和环状RNA-0134944的失调与中国汉族绝经后女性骨质疏松症及骨质疏松性骨折易感性相关。
Pharmgenomics Pers Med. 2023 Mar 10;16:183-194. doi: 10.2147/PGPM.S394757. eCollection 2023.
2
EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression.EIF4A3 调控的 circ_0087429 通过 miR-5003-3p 依赖性上调 OGN 表达逆转 EMT 并抑制宫颈癌进展。
J Exp Clin Cancer Res. 2022 May 5;41(1):165. doi: 10.1186/s13046-022-02368-4.
3
Overexpression of circ_0021739 in Peripheral Blood Mononuclear Cells in Women with Postmenopausal Osteoporosis Is Associated with Reduced Expression of microRNA-194-5p in Osteoclasts.circ_0021739 在绝经后骨质疏松症女性外周血单个核细胞中的过表达与破骨细胞中 microRNA-194-5p 的表达降低有关。
Med Sci Monit. 2021 Apr 20;27:e929170. doi: 10.12659/MSM.929170.
4
Circular RNA hsa_circ_0069117 suppresses proliferation and migration of osteosarcoma cells lines via miR-875-3p/PF4V1 axis.环状 RNA hsa_circ_0069117 通过 miR-875-3p/PF4V1 轴抑制骨肉瘤细胞系的增殖和迁移。
J Orthop Surg Res. 2022 Jan 21;17(1):37. doi: 10.1186/s13018-022-02923-x.
5
Analysis of Expression Profiles of CircRNA and MiRNA in Oviduct during the Follicular and Luteal Phases of Sheep with Two Fecundity ( Gene) Genotypes.具有两种繁殖力（基因）基因型的绵羊卵泡期和黄体期输卵管中circRNA和miRNA表达谱分析
Animals (Basel). 2021 Sep 28;11(10):2826. doi: 10.3390/ani11102826.
6
Identification of Serum Exosome-Derived circRNA-miRNA-TF-mRNA Regulatory Network in Postmenopausal Osteoporosis Using Bioinformatics Analysis and Validation in Peripheral Blood-Derived Mononuclear Cells.基于生物信息学分析和外周血单核细胞验证鉴定绝经后骨质疏松症血清外泌体来源 circRNA-miRNA-TF-mRNA 调控网络
Front Endocrinol (Lausanne). 2022 Jun 9;13:899503. doi: 10.3389/fendo.2022.899503. eCollection 2022.
7
circ-PTK2 (hsa_circ_0008305) regulates the pathogenic processes of ovarian cancer via miR-639 and FOXC1 regulatory cascade.环状PTK2（hsa_circ_0008305）通过miR-639和FOXC1调控级联反应调节卵巢癌的致病过程。
Cancer Cell Int. 2021 May 25;21(1):277. doi: 10.1186/s12935-021-01985-x.
8
Identification of circ_0000375 and circ_0011536 as novel diagnostic biomarkers of colorectal cancer.鉴定circ_0000375和circ_0011536作为结直肠癌的新型诊断生物标志物。
World J Clin Cases. 2022 Apr 16;10(11):3352-3368. doi: 10.12998/wjcc.v10.i11.3352.
9
Circular RNA hsa_circ_0076906 competes with OGN for miR-1305 biding site to alleviate the progression of osteoporosis.环状 RNA hsa_circ_0076906 通过与 OGN 竞争 miR-1305 结合位点来缓解骨质疏松症的进展。
Int J Biochem Cell Biol. 2020 May;122:105719. doi: 10.1016/j.biocel.2020.105719. Epub 2020 Feb 19.
10
Hsa_circ_0006404 and hsa_circ_0000735 Regulated Ovarian Cancer Response to Docetaxel Treatment via Regulating p-GP Expression.Hsa_circ_0006404 和 hsa_circ_0000735 通过调节 p-GP 表达调控卵巢癌细胞对多西他赛治疗的反应。
Biochem Genet. 2022 Feb;60(1):395-414. doi: 10.1007/s10528-021-10080-9. Epub 2021 Jul 13.

引用本文的文献

1
hsa_circ_0004276 inhibits osteogenic differentiation of bone marrow mesenchymal stem cells and exacerbates postmenopausal osteoporosis through interaction with ELAVL1.hsa_circ_0004276通过与ELAVL1相互作用抑制骨髓间充质干细胞的成骨分化并加重绝经后骨质疏松症。
Clinics (Sao Paulo). 2025 Aug 12;80:100718. doi: 10.1016/j.clinsp.2025.100718.
2
Exploring the Role of Circular RNA in Bone Biology: A Comprehensive Review.探索环状 RNA 在骨生物学中的作用：全面综述。
Cells. 2024 Jun 7;13(12):999. doi: 10.3390/cells13120999.
3
CircCOX6A1 suppresses osteogenic differentiation and aggravates osteoporosis via miR-512-3p/DYRK2 axis.

本文引用的文献

1
Circ_0134944 inhibits osteogenesis through miR-127-5p/PDX1/SPHK1 pathway.Circ_0134944通过miR-127-5p/PDX1/SPHK1通路抑制成骨作用。
Regen Ther. 2021 Sep 29;18:391-400. doi: 10.1016/j.reth.2021.09.004. eCollection 2021 Dec.
2
Circular RNA hsa_circ_0076906 competes with OGN for miR-1305 biding site to alleviate the progression of osteoporosis.环状 RNA hsa_circ_0076906 通过与 OGN 竞争 miR-1305 结合位点来缓解骨质疏松症的进展。
Int J Biochem Cell Biol. 2020 May;122:105719. doi: 10.1016/j.biocel.2020.105719. Epub 2020 Feb 19.
3
circRNA_0016624 could sponge miR-98 to regulate BMP2 expression in postmenopausal osteoporosis.
环状 RNA COX6A1 通过 miR-512-3p/DYRK2 轴抑制成骨分化并加重骨质疏松症。
Mol Biol Rep. 2024 May 10;51(1):636. doi: 10.1007/s11033-024-09532-3.
4
The critical role of toll-like receptor 4 in bone remodeling of osteoporosis: from inflammation recognition to immunity.Toll 样受体 4 在骨质疏松症骨重建中的关键作用：从炎症识别到免疫。
Front Immunol. 2024 Mar 5;15:1333086. doi: 10.3389/fimmu.2024.1333086. eCollection 2024.
circRNA_0016624 可以通过海绵吸附 miR-98 来调节绝经后骨质疏松症中的 BMP2 表达。
Biochem Biophys Res Commun. 2019 Aug 20;516(2):546-550. doi: 10.1016/j.bbrc.2019.06.087. Epub 2019 Jun 22.
4
miR-384-5p Targets and Negatively Regulates Age-Related Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells.miR-384-5p 靶向并负调控大鼠骨髓间充质干细胞的衰老相关成骨分化。
Stem Cells Dev. 2019 Jun 15;28(12):791-798. doi: 10.1089/scd.2019.0044. Epub 2019 May 20.
5
Circular RNA and its mechanisms in disease: From the bench to the clinic.环状 RNA 及其在疾病中的机制：从实验室到临床。
Pharmacol Ther. 2018 Jul;187:31-44. doi: 10.1016/j.pharmthera.2018.01.010. Epub 2018 Feb 14.
6
Effects of Osteoglycin (OGN) on treating senile osteoporosis by regulating MSCs.骨甘蛋白（OGN）通过调节间充质干细胞治疗老年性骨质疏松症的作用
BMC Musculoskelet Disord. 2017 Oct 26;18(1):423. doi: 10.1186/s12891-017-1779-7.
7
Osteoporosis: Staying strong.骨质疏松症：保持骨骼强健。
Nature. 2017 Oct 4;550(7674):S15-S17. doi: 10.1038/550S15a.
8
Osteoporosis.骨质疏松症。
Ann Intern Med. 2017 Aug 1;167(3):ITC17-ITC32. doi: 10.7326/AITC201708010.
9
Osteoporosis treatment: recent developments and ongoing challenges.骨质疏松症治疗：最新进展和持续挑战。
Lancet Diabetes Endocrinol. 2017 Nov;5(11):898-907. doi: 10.1016/S2213-8587(17)30188-2. Epub 2017 Jul 7.
10
Long-term mipomersen treatment is associated with a reduction in cardiovascular events in patients with familial hypercholesterolemia.长期使用米泊美生治疗与家族性高胆固醇血症患者心血管事件的减少有关。
J Clin Lipidol. 2016 Jul-Aug;10(4):1011-1021. doi: 10.1016/j.jacl.2016.04.013. Epub 2016 May 9.