Clinical Research Center, Masan National Tuberculosis Hospital, Changwon, Republic of Korea.
Department of Internal Medicine, Division of Pulmonology, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, Republic of Korea.
Int J Mycobacteriol. 2023 Jan-Mar;12(1):66-72. doi: 10.4103/ijmy.ijmy_12_23.
Multiple attempts have been made to use biological samples other than sputum to diagnose tuberculosis (TB). Sputum acid-fast bacillus (AFB) microscopy is the fastest, most straightforward, and most inexpensive method for diagnosing pulmonary TB. However, urine can be used in place of sputum owing to its various advantages, such as a noninvasive method of collection, convenient handling and storage, and minimal risk of infection in health-care workers involved in sample collection. In this study, we aimed to assess the suitability of urine as a sample to obtain transrenal DNA (trDNA) to diagnose TB. This study involved several patients with TB undergoing inpatient treatment, whose AFB microscopy showed negative inversion.
Here, 51 urine samples were collected from 40 patients with TB and examined to confirm the presence of trDNA. First, we compared the efficiency of two trDNA extraction methods.
An automated magnetic bead-based method and a more efficient anchoring extraction method. Statistical analyses were performed using Excel software (Microsoft Office Professional Plus 2019).
Although molecular diagnosis using GeneXpert yielded negative results, a peculiarity was observed. There was no significant difference between GeneXpert findings and our results nor was there any difference in the sequential trDNA samples obtained. However, even when GeneXpert results were negative, trDNA was detected in seven out of ten samples using the anchor extraction method.
Further studies are needed to establish biomarkers for the progression of TB treatment.
人们曾多次尝试使用痰液以外的生物样本来诊断结核病（TB）。痰抗酸杆菌（AFB）显微镜检查是诊断肺结核（PTB）最快、最直接、最便宜的方法。然而，由于尿液具有采集方便、处理和储存方便、采集样本的医护人员感染风险极小等优点，因此可以替代痰液。在本研究中，我们旨在评估尿液作为获取转肾 DNA（trDNA）以诊断结核病的样本的适用性。这项研究涉及了一些接受住院治疗的结核病患者，他们的 AFB 显微镜检查结果为阴性反转。
从 40 例结核病患者中采集了 51 份尿液样本，并对其 trDNA 进行了检测。首先，我们比较了两种 trDNA 提取方法的效率。
一种自动化的基于磁珠的方法和一种更有效的锚定提取方法。统计分析使用 Excel 软件（Microsoft Office Professional Plus 2019）进行。
尽管使用 GeneXpert 进行分子诊断的结果为阴性，但观察到一个特点。GeneXpert 的检测结果与我们的结果之间没有显著差异，也没有在连续的 trDNA 样本中发现差异。然而，即使 GeneXpert 的结果为阴性，使用锚定提取方法也可以从十个样本中的七个样本中检测到 trDNA。
需要进一步研究来建立结核病治疗进展的生物标志物。
