Chen Hao, Sun Yishuo, Liu Meihui, Li Fuyou, Peng Qian, Huang Hui
College of Materials Science and Opto-Electronic Technology & Center of Materials Science and Optoelectronics Engineering & CAS Center for Excellence in Topological Quantum Computation & CAS Key Laboratory of Vacuum Physic, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Department of Chemistry & State Key Laboratory of Molecular Engineering of Polymers & Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, 2005 Songhu Road, Shanghai, 200438, P. R. China.
Angew Chem Int Ed Engl. 2023 May 8;62(20):e202302629. doi: 10.1002/anie.202302629. Epub 2023 Apr 12.
The dual-ratiometric thermometry is one of highly accurate methods for microscopic thermal measurement in biological systems. Herein, a series of chromone derivatives with noncovalently intramolecular interactions (NIIs) were designed and synthesized for ratiometric thermometers. The triplet states of these organic compounds were systematically tuned upon regulating the conformation with NIIs to yield efficient room temperature phosphorescence and large wavelength difference between fluorescence and phosphorescence simultaneously. As a result, an unprecedent organic 3D dual-ratiometric thermometer was established based on the intensity ratio and lifetime ratio of fluorescence/phosphorescence vs temperature, which was used for in vitro and in vivo bio-thermometry with high accuracy. This work provides a novel method to achieve organic dual ratiometric thermometers via tuning the triplet excited states.
双比率测温法是生物系统中微观热测量的高精度方法之一。在此，设计并合成了一系列具有非共价分子内相互作用（NIIs）的色酮衍生物用于比率温度计。通过用NIIs调节构象，系统地调整了这些有机化合物的三重态，以同时产生有效的室温磷光以及荧光和磷光之间的大波长差。结果，基于荧光/磷光强度比和寿命比与温度的关系，建立了一种前所未有的有机3D双比率温度计，该温度计用于体外和体内生物测温，具有很高的精度。这项工作提供了一种通过调节三重激发态来实现有机双比率温度计的新方法。
