Ogo Fernanda Mithie, Siervo Glaucia Eloisa Munhoz Lion, Praxedes Ana Maria, Vieira Henrique Rodrigues, da Silva Scarton Suellen Ribeiro, Bitencourt Ana Tereza Guimarães, Arena Arielle Cristina, Simão Andréa Name Colado, Guerra Marina Trevizan, de Freitas Mathias Paulo Cesar, Fernandes Glaura Scantamburlo Alves
Department of General Biology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Brazil.
Department of Pathological Sciences, Biological Sciences Center, State University of Londrina - UEL, Londrina, Brazil.
Birth Defects Res. 2023 Apr 15;115(7):710-721. doi: 10.1002/bdr2.2161. Epub 2023 Mar 17.
It has been suggested that maternal exposure to constant light during the gestational period could be considered as a chronic stressor, impairing offspring development by interfering in neuroendocrine and behavior responses.
This study aimed to evaluate whether maternal exposure to continuous light during pregnancy affects the adult reproductive system in the female offspring.
Pregnant Wistar rats were allocated into light-dark (LD) group, exposed to light and dark photoperiod during gestation, and the light-light (LL) group, exposed to a photoperiod of constant light during gestation. After birth, pups were maintained under normal light-dark photoperiod until adulthood. At postnatal day 90, blood was collected from the female offspring, to analyze plasma luteinizing hormone (LH) and progesterone levels, and the uterus and ovaries were harvested for morphometric, histological, and oxidative stress evaluations.
Female exposure to continuous light during the intrauterine period resulted in the adult reduction of LH and increased progesterone plasma levels, and uterine injuries a higher number of endometrial glands and reduced levels of antioxidant enzymes, such as glutathione reductase and glutathione S-transferase. In these experimental conditions, gestational continuous light exposure disturbs sex hormone balance and reduces the antioxidant enzymatic activity in the uterus of female offspring in adult life.
有人提出，孕期母体持续暴露于光照下可被视为一种慢性应激源，通过干扰神经内分泌和行为反应来损害后代发育。
本研究旨在评估孕期母体暴露于持续光照是否会影响雌性后代的成年生殖系统。
将怀孕的Wistar大鼠分为明暗（LD）组，在妊娠期暴露于明暗光周期，以及光照（LL）组，在妊娠期暴露于持续光照周期。出生后，幼崽在正常明暗光周期下饲养至成年。在出生后第90天，从雌性后代采集血液，分析血浆促黄体生成素（LH）和孕酮水平，并采集子宫和卵巢进行形态计量学、组织学和氧化应激评估。
子宫内期雌性暴露于持续光照导致成年后LH降低、血浆孕酮水平升高，以及子宫损伤——子宫内膜腺体数量增加，抗氧化酶如谷胱甘肽还原酶和谷胱甘肽S-转移酶水平降低。在这些实验条件下，孕期持续光照暴露会扰乱成年雌性后代子宫中的性激素平衡并降低抗氧化酶活性。
