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豆粕的诱导非酶褐变。II. 用木糖处理的大豆蛋白在瘤胃中的逃逸及门静脉净吸收

Induced non-enzymatic browning of soybean meal. II. Ruminal escape and net portal absorption of soybean protein treated with xylose.

作者信息

Cleale R M, Britton R A, Klopfenstein T J, Bauer M L, Harmon D L, Satterlee L D

机构信息

Dept. of Anim. Sci., University of Nebraska, Lincoln 68586-0908.

出版信息

J Anim Sci. 1987 Nov;65(5):1319-26. doi: 10.2527/jas1987.6551319x.

DOI:10.2527/jas1987.6551319x
PMID:3693157
Abstract

Non-enzymatic browning was tested as a means of increasing ruminal escape of soybean meal N. Soybean meal was treated with xylose (3 mol/mol SBM-lysine), sodium hydroxide (pH 8.5) and enough water to achieve an 83% dry matter mixture and then heated at 150 C for 30 min (XTS-30). Trial 1 evaluated ruminal escape of N from XTS-30 compared with commercial soybean meal (CS) or urea (U) in a replicated 3 X 3 Latin square design using six duodenally cannulated Angus X Hereford steers (24.7 kg). Duodenal flow of dietary N was higher (P less than for steers fed XTS-30 (47.9 g/d) than for steers fed CS (39.5 g/d). The ruminal escape estimate for XTS-30 (33.7%) was higher (P less than .10) than CS (13.1%), whereas total tract apparent N digestibility was not different among treatments. In trial 2, net portal absorption of alpha-amino N was measured in Finnsheep X Suffolk ram lambs (24.7 kg) fed U, CS or XTS-30 in a 3 X 3 Latin square design. Portal blood flow was measured by primed, continuous infusion of para-aminohippuric acid. Portal blood flow was lower (P less than .05) for U.fed lambs than for lambs fed CS or XTS-30, and tended to be lower for lambs fed CS than those fed XTS-30. Net portal absorption of alpha-amino N tended to be lowest for lambs fed U (281 mmol/d) and highest for lambs fed XTS-30 (578 mmol/d). The results are interpreted to show that non-enzymatic browning increased flow of soybean meal N to the intestine.

摘要

非酶褐变被作为一种增加豆粕氮瘤胃逃逸率的方法进行了测试。豆粕用木糖(3摩尔/摩尔豆粕赖氨酸)、氢氧化钠(pH 8.5)和足够的水进行处理,以达到83%的干物质混合物,然后在150℃下加热30分钟(XTS - 30)。试验1采用重复的3×3拉丁方设计,使用6头十二指肠插管的安格斯×赫里福德阉牛(24.7千克),评估了XTS - 30与商业豆粕(CS)或尿素(U)相比氮的瘤胃逃逸情况。饲喂XTS - 30的阉牛(47.9克/天)的日粮氮十二指肠流量高于(P小于)饲喂CS的阉牛(39.5克/天)。XTS - 30的瘤胃逃逸估计值(33.7%)高于(P小于0.10)CS(13.1%),而各处理间全消化道表观氮消化率无差异。在试验2中,采用3×3拉丁方设计,在饲喂U、CS或XTS - 30的芬兰羊×萨福克公羊羔(24.7千克)中测量了α-氨基氮的净门静脉吸收量。通过灌注对氨基马尿酸的连续灌注法测量门静脉血流量。饲喂U的羔羊的门静脉血流量低于(P小于0.05)饲喂CS或XTS - 30的羔羊,并且饲喂CS的羔羊的门静脉血流量往往低于饲喂XTS - 30的羔羊。饲喂U的羔羊的α-氨基氮净门静脉吸收量往往最低(281毫摩尔/天),而饲喂XTS - 30的羔羊最高(578毫摩尔/天)。结果表明,非酶褐变增加了豆粕氮向肠道的流量。

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