用于研究髓鞘形成的人诱导多能干细胞衍生的少突胶质细胞谱系细胞与神经元的三维共培养平台
Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination.
作者信息
von der Bey Martina, De Cicco Silvia, Zach Susanne, Hengerer Bastian, Ercan-Herbst Ebru
机构信息
BioMed X Institute, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany; Molecular and Translational Neuroscience, Department of Neurology, Ulm University, N27, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
BioMed X Institute, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
出版信息
STAR Protoc. 2023 Mar 17;4(2):102164. doi: 10.1016/j.xpro.2023.102164.
Developing an in vitro platform to study neuron-oligodendrocyte interaction, particularly myelination, is essential to understand aberrant myelination in neuropsychiatric and neurodegenerative diseases. Here, we provide a controlled, direct co-culture protocol for human induced-pluripotent-stem-cell (hiPSC)-derived neurons and oligodendrocytes on three-dimensional (3D) nanomatrix plates. We describe steps to differentiate hiPSCs into cortical neurons and oligodendrocyte lineage cells on 3D nanofibers. We then detail the detachment and isolation of the oligodendrocyte lineage cells, followed by neuron-oligodendrocyte co-culture in this 3D microenvironment.
开发一个体外平台来研究神经元-少突胶质细胞相互作用，尤其是髓鞘形成，对于理解神经精神疾病和神经退行性疾病中的异常髓鞘形成至关重要。在此，我们提供了一种在三维（3D）纳米基质板上对人诱导多能干细胞（hiPSC）衍生的神经元和少突胶质细胞进行可控、直接共培养的方案。我们描述了在3D纳米纤维上将hiPSC分化为皮质神经元和少突胶质细胞谱系细胞的步骤。然后，我们详细介绍了少突胶质细胞谱系细胞的分离和分离过程，随后在这个3D微环境中进行神经元-少突胶质细胞共培养。
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