亚油酸刺激可导致体外产生转化生长因子-β1并抑制猪流行性腹泻病毒感染。
Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro.
作者信息
Yang Shanshan, Wang Caiying, Huang Xin, Jansen Christine A, Savelkoul Huub F J, Liu Guangliang
机构信息
State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, China; Cell Biology and Immunology Group, Wageningen University and Research, Wageningen, the Netherlands.
State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, China.
出版信息
Virology. 2023 Apr;581:89-96. doi: 10.1016/j.virol.2023.03.004. Epub 2023 Mar 14.
Linoleic acid (LA) is recommended to improve pork quality. However, whether it affects the intestinal immune response in pigs is still unclear. Our ex vivo experiments demonstrated that LA stimulation resulted in increased frequencies of Tregs in PBMCs but not in Peyer's Patches (PPs). The results of RT-qPCR, flow cytometry, and ELISA indicated that LA increased the TGF-β1 expression level in DCs isolated from PEDV infected pigs. Furthermore, RT-qPCR and flow cytometry results demonstrated that TGF-β1 was associated with higher frequencies of Tregs both in PBMCs and PPs. Additional investigations showed that TGF-β1 inhibited PEDV infection in vitro. Besides, knocking-out TGF-β1 in IPEC-J2 cells resulted in higher viral load. Taken together, our results demonstrated that LA stimulation resulted in enhanced production of TGF-β1 by DC, which resulted in higher frequencies of Tregs production and inhibition of PEDV infection.
推荐亚油酸（LA）来改善猪肉品质。然而，其是否影响猪的肠道免疫反应仍不清楚。我们的体外实验表明，LA刺激导致外周血单核细胞（PBMCs）中调节性T细胞（Tregs）频率增加，但派伊尔结（PPs）中未增加。逆转录定量聚合酶链反应（RT-qPCR）、流式细胞术和酶联免疫吸附测定（ELISA）结果表明，LA提高了从感染猪流行性腹泻病毒（PEDV）的猪分离出的树突状细胞（DCs）中转化生长因子-β1（TGF-β1）的表达水平。此外，RT-qPCR和流式细胞术结果表明，TGF-β1与PBMCs和PPs中更高频率的Tregs相关。进一步研究表明，TGF-β1在体外抑制PEDV感染。此外，在肠上皮细胞系-J2（IPEC-J2）细胞中敲除TGF-β1导致病毒载量更高。综上所述，我们的结果表明，LA刺激导致DC产生TGF-β1增加，从而导致Tregs产生频率更高并抑制PEDV感染。
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