Fractionation of plant and animal high mobility group chromosomal proteins by ion-exchange and reversed-phase high-performance liquid chromatography.
作者信息
Spiker S, Bates M, Arwood L J
机构信息
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
出版信息
J Chromatogr. 1987 Sep 18;405:327-36. doi: 10.1016/s0021-9673(01)81774-x.
PMID:3693471
Abstract
A method for purifying wheat high mobility group (HMG) chromosomal proteins using a combination of weak cation-exchange and reversed-phase high-performance liquid chromatography (HPLC) is described. Previously reported HPLC systems devised for fractionating HMG proteins of vertebrate animals are not effective for the plant proteins. The system described here can be used for the fractionation of both plant and animal HMG proteins.
摘要
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引用本文的文献
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DNA binding mediated by the wheat HMGa protein: a novel instance of selectivity against alternating GC sequence.小麦HMGa蛋白介导的DNA结合：对交替GC序列具有选择性的新实例。
Plant Mol Biol. 2001 May;46(2):193-204. doi: 10.1023/a:1010696604330.
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High mobility group chromosomal proteins bind to AT-rich tracts flanking plant genes.高迁移率族染色体蛋白与植物基因侧翼富含AT的区域结合。
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