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来自鸡血小板和红细胞的高迁移率族非组蛋白染色体蛋白。

High mobility group non-histone chromosomal proteins from chicken thrombocytes and erythrocytes.

作者信息

Krajewska W M, Kłyszejko-Stefanowicz L

出版信息

Acta Biochim Pol. 1985;32(2):87-94.

PMID:4036451
Abstract

The high mobility group non-histone chromosomal proteins (HMG) from chicken thrombocytes were compared with those from chicken erythrocytes. In terms of their extractability, electrophoretic behaviour in polyacrylamide gels containing 2.5 M-urea, and amino acid composition, the HMG proteins of these cells were indistinguishable. However, microcomplement fixation assay performed in the presence of antiserum against HMG-1 and HMG-2 revealed that these high molecular weight HMG proteins of chicken thrombocytes and erythrocytes are not cross-reactive. Electrophoretic analysis of HMG-1 and HMG-2 from these two types of cells in polyacrylamide gels containing 6.25 M-urea suggested differences in the HMG-2 patterns.

摘要

对来自鸡血小板的高迁移率族非组蛋白染色体蛋白(HMG)与来自鸡红细胞的HMG进行了比较。就其可提取性、在含2.5M尿素的聚丙烯酰胺凝胶中的电泳行为以及氨基酸组成而言,这些细胞的HMG蛋白无法区分。然而,在存在抗HMG - 1和HMG - 2抗血清的情况下进行的微量补体结合试验表明,鸡血小板和红细胞的这些高分子量HMG蛋白没有交叉反应性。在含6.25M尿素的聚丙烯酰胺凝胶中对这两种细胞的HMG - 1和HMG - 2进行电泳分析,结果显示HMG - 2的图谱存在差异。

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