Prakasham Karthikeyan, Gurrani Swapnil, Shiea Jentaie, Wu Ming-Tsang, Wu Chia-Fang, Lin Yu-Chia, Tsai Bongee, Huang Po-Chin, Andaluri Gangadhar, Ponnusamy Vinoth Kumar
PhD Program in Environmental and Occupational Medicine (College of Medicine), & Research Center for Precision Environmental Medicine, Kaohsiung Medical University (KMU), Kaohsiung City 807, Taiwan.
Department of Medicinal and Applied Chemistry, Kaohsiung Medical University (KMU), Kaohsiung City 807, Taiwan; Department of Chemistry, National Sun Yat-Sen University, Kaohsiung City 804, Taiwan.
Food Chem. 2023 Aug 15;417:135951. doi: 10.1016/j.foodchem.2023.135951. Epub 2023 Mar 15.
In this study, we demonstrated a novel semi-automated in-syringe-based coagulant-assisted liquid-liquid microextraction (IS-CGA-LLME) as fast mycotoxin extraction (FaMEx) technique coupled with ultra-high-performance liquid chromatography connected with a tandem-mass spectrometer (UHPLC-MS/MS) for the quantification of mycotoxin (Ochratoxin A, OT-A) in coffee and tea samples. IS-CGA-LLME is a three-step extraction process that includes extraction of OT-A from sample matrix using low-volume solvent extraction, then the extractant was cleaned-up using a coagulation process, and finally, the decolorized/matrix removed sample solution was processed for LLME for target analyte's pre-concentration. The final extractant was analyzed using UHPLC-MS/MS for OT-A quantification. Under the optimized experimental conditions, highly sensitive detection and quantification limits were obtained at 0.001 and 0.003 ng g for OT-A with excellent extraction recovery (93-111%) and precision <10%. These results proved that the developed method is a simple, highly sensitive, semi-automated, low-matrix effect and efficient procedure for the determination of mycotoxins in food samples.
在本研究中，我们展示了一种新型的基于注射器的半自动凝固剂辅助液-液微萃取（IS-CGA-LLME）快速霉菌毒素萃取（FaMEx）技术，该技术与超高效液相色谱-串联质谱仪（UHPLC-MS/MS）联用，用于定量咖啡和茶样品中的霉菌毒素（赭曲霉毒素A，OT-A）。IS-CGA-LLME是一个三步萃取过程，包括使用小体积溶剂萃取从样品基质中萃取OT-A，然后通过凝固过程对萃取剂进行净化，最后，对脱色/去除基质的样品溶液进行液-液微萃取以预浓缩目标分析物。使用UHPLC-MS/MS对最终萃取剂进行分析以定量OT-A。在优化的实验条件下，OT-A的检测限和定量限分别为0.001和0.003 ng/g，萃取回收率极佳（93-111%），精密度<10%。这些结果证明，所开发的方法是一种简单、高灵敏度、半自动、低基质效应且高效的食品样品中霉菌毒素测定方法。
