Department of Applied Chemistry, National Yang Ming Chiao Tung University, 1001 University Rd., Hsinchu, 300, Taiwan; Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Rd., Hsinchu, 300044, Taiwan.
Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Rd., Hsinchu, 300044, Taiwan.
Anal Chim Acta. 2023 Apr 29;1252:341028. doi: 10.1016/j.aca.2023.341028. Epub 2023 Feb 27.
A facile and rapid skin metabolomics protocol is proposed. The liquid microjunction-surface sampling probe system has been partly automated, and used in conjunction with hydrogel probes for skin metabolite analysis. A control device was built to precisely control the segmented solvent flow and analyte re-extraction into the liquid microjunction. This mode provides rapid online re-extraction of the analytes from hydrogel probes. Humectant was added to the hydrogel, and moist heat treatment was used to make the hydrogel probes rugged for sampling in the clinical setting. The developed method was validated for the analysis of choline - a putative biomarker of psoriasis. A linear relationship over six calibration levels from 3.18 × 10 to 3.18 × 10 mol m has been obtained. The limit of detection was 6.6 × 10 mol m, while the recoveries range from 92 to 109%. The within-run and between-run precision were evaluated and the coefficients of variation range from 1.84 to 7.13%. Furthermore, the developed method has been used to screen patients (n = 10) and healthy participants (control group; n = 10) for psoriasis-related skin metabolites. Metabolomic profiling of the skin excretion-related signals identified potential biomarkers of psoriasis: choline, pipecolic acid, ornithine, urocanic acid, and methionine.
提出了一种简便快速的皮肤代谢组学方法。已经部分实现了液体微连接-表面采样探针系统的自动化，并与水凝胶探针一起用于皮肤代谢物分析。构建了一个控制装置，以精确控制分段溶剂流和分析物重新提取到液体微连接中。这种模式提供了从水凝胶探针中快速在线重新提取分析物。在水凝胶中添加保湿剂，并进行湿热处理，使水凝胶探针坚固耐用，可用于临床采样。该方法已用于分析胆碱（银屑病的潜在生物标志物）进行了验证。已经获得了从 3.18×10 到 3.18×10 摩尔 m 的六个校准水平的线性关系。检测限为 6.6×10 摩尔 m，回收率范围为 92%至 109%。评估了批内和批间精密度，变异系数范围为 1.84%至 7.13%。此外，该方法已用于筛选银屑病患者（n=10）和健康参与者（对照组；n=10）的皮肤代谢物。皮肤排泄相关信号的代谢组学分析确定了银屑病的潜在生物标志物：胆碱、哌啶酸、鸟氨酸、尿刊酸和蛋氨酸。
