Watts J L, Owens W E
Mastitis Research Laboratory, Louisiana State University Agricultural Center, Homer 71040.
J Clin Microbiol. 1987 Nov;25(11):2037-9. doi: 10.1128/jcm.25.11.2037-2039.1987.
A total of 353 coagulase-negative staphylococcus (CNS) isolates from infected bovine mammary glands were tested for cytolysin production by using the synergistic hemolysin assay (SHA). Overall, 34.6% of CNS isolates were SHA positive. Human-associated, coagulase-negative staphylococcal species contained the greatest number of SHA-positive strains. Milk leukocyte levels expressed as somatic cell counts (SCC) were elevated with SHA-positive Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus warneri strains. Elevated SCC levels were associated with strains of Staphylococcus hyicus and Staphylococcus chromogenes. However, no difference in SCC levels was observed between SHA-positive and SHA-negative strains. Results indicated that the SHA was a sensitive test for the detection of cytolysin-producing CNS.
通过协同溶血素检测法(SHA)对从感染的牛乳腺中分离出的353株凝固酶阴性葡萄球菌(CNS)进行了溶细胞素产生检测。总体而言,34.6%的CNS分离株SHA检测呈阳性。与人类相关的凝固酶阴性葡萄球菌种类中,SHA阳性菌株数量最多。以体细胞计数(SCC)表示的乳白细胞水平在SHA阳性的表皮葡萄球菌、人葡萄球菌和沃氏葡萄球菌菌株中有所升高。SCC水平升高与猪葡萄球菌和产色葡萄球菌菌株有关。然而,SHA阳性和阴性菌株之间在SCC水平上未观察到差异。结果表明,SHA是检测产生溶细胞素的CNS的一种灵敏检测方法。