Animal Physiology Laboratory, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
Department of Life Sciences, High Teacher Training College of Bertoua, University of Bertoua, Bertoua, Cameroon.
BMC Endocr Disord. 2023 Mar 20;23(1):64. doi: 10.1186/s12902-022-01252-8.
Diabetes mellitus is a metabolic disease characterized by an abnormally high blood glucose level. Glucose intolerance and insulin resistance are two characteristics that promote the onset and development of type 2 diabetes. The aim of this study was to create a diabetic rat model from obese rat MACAPOS 2.
A group of rats was subjected to a high-fat diet (HFD) compared to a control group (NC) which received a normal diet. After 16 weeks of HFD, Lee index was calculated, obese rats were subjected to an oral glucose tolerance test (OGTT) and insulin tolerance test (ITT). One group of HFD rats (HFDZ) received streptozotocin 22.5 mg/kg (iv). One week later, weight gain, water and food intakes, urine volume and fasting blood glucose levels were evaluated. Animals were also subjected to glucose tolerance and insulin tolerance tests.
After 16 weeks of HFD, rats became obese, glucose intolerant and resistant to insulin. The body weight of rats was significantly high (+ 26.23%) compared to normal rats, glycemia remained significantly high (+ 45.46%, P < 0.01) two hours after administration of glucose in high-fat diet rats, water intake and urine volume were comparable to those of NC. In HFD, the streptozotocin injected after one week (HFDZ), amplified glucose intolerance. During ITT, glycemia remained significantly (P < 0.01) high from 15 min; and did not vary during the 60 min of ITT. The fasting glycemia one week after streptozotocin injection was significantly high (288 mg/dL) compared to HFD (114 mg/dL), associated whit a significant (P < 0.01) increase in water intake and 24 h urine volume.
These results showed that MACAPOS 2 associated with a low dose of streptozotocin (22.5 mg/dL) early leads to the diabetes in obese albinos Wistar rats and could be a real model to study the type 2 diabetes mellitus.
糖尿病是一种代谢疾病，其特征是血糖水平异常升高。葡萄糖耐量和胰岛素抵抗是促进 2 型糖尿病发病和发展的两个特征。本研究旨在从肥胖大鼠 MACAPOS 2 中建立糖尿病大鼠模型。
一组大鼠给予高脂肪饮食（HFD），与给予正常饮食的对照组（NC）进行比较。HFD 16 周后，计算 Lee 指数，肥胖大鼠行口服葡萄糖耐量试验（OGTT）和胰岛素耐量试验（ITT）。一组 HFD 大鼠（HFDZ）给予链脲佐菌素 22.5mg/kg（iv）。一周后，评估体重增加、水和食物摄入量、尿量和空腹血糖水平。动物还进行了葡萄糖耐量和胰岛素耐量试验。
HFD 16 周后，大鼠肥胖、葡萄糖耐量降低且对胰岛素抵抗。与正常大鼠相比，HFD 大鼠体重显著升高（+26.23%），葡萄糖负荷后 2 小时血糖仍显著升高（+45.46%，P<0.01），HFD 大鼠的水摄入量和尿量与 NC 大鼠相似。在 HFD 中，一周后注射的链脲佐菌素（HFDZ）放大了葡萄糖耐量不良。在 ITT 中，血糖从 15 分钟开始一直显著升高（P<0.01），并且在 ITT 的 60 分钟内没有变化。注射链脲佐菌素一周后空腹血糖显著升高（288mg/dL），与 HFD（114mg/dL）相比，水摄入量和 24 小时尿量显著增加（P<0.01）。
这些结果表明，MACAPOS 2 联合低剂量链脲佐菌素（22.5mg/dL）早期导致肥胖白化 Wistar 大鼠糖尿病，可能是研究 2 型糖尿病的真实模型。
