Iyengar L, Rao A V
Biotechnol Bioeng. 1979 Aug;21(8):1333-43. doi: 10.1002/bit.260210803.
The enzyme urease (urea amidohydrolase, EC 3.5.1.5) prepared from Cajanus indicus, has been immobilized with glutaraldehyde-treated chitin as the solid support. The immobilized enzyme was characterized by determining the pH profiles and optimum temperature. Effect of glutaraldehyde concentration on the binding of enzyme to chitin was studied. The storage stability of the chitin-urease system was determined.
从木豆中制备的脲酶（尿素酰胺水解酶，EC 3.5.1.5）已以经戊二醛处理的几丁质作为固体载体进行固定化。通过测定pH曲线和最适温度对固定化酶进行了表征。研究了戊二醛浓度对酶与几丁质结合的影响。测定了几丁质-脲酶系统的储存稳定性。
