hexanucleotide repeat allele tagging SNPs: Associations with ALS risk and longevity.

hexanucleotide repeat expansion is a common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The locus may harbor residual risk outside the hexanucleotide repeat expansion, but the evidence is conflicting. Here, we first compared 683 unrelated amyotrophic lateral sclerosis cases and 3,196 controls with Finnish ancestry to find best single nucleotide polymorphisms that tag the hexanucleotide repeat expansion and intermediate-length alleles. Rs2814707 was the best tagging single nucleotide polymorphisms for intermediate-length alleles with ≥7 repeats ( = 5 × 10) and rs139185008 for the hexanucleotide repeat expansion ( = 7 × 10) as well as alleles with ≥20 repeats. rs139185008C associated with amyotrophic lateral sclerosis after removing cases with the hexanucleotide repeat expansion, especially in the subpopulation homozygous for the rs2814707T ( = 0.0002, OR = 5.06), which supports the concept of residual amyotrophic lateral sclerosis risk at the haplotypes other than the hexanucleotide repeat expansion. We then leveraged Finnish biobank data to test the effects of rs2814707T and rs139185008C on longevity after removing individuals with amyotrophic lateral sclerosis / frontotemporal dementia diagnoses. In the discovery cohort ( = 230,006), the frequency of rs139185008C heterozygotes decreased significantly with age in the comparisons between 50 and 80 years vs. >80 years ( = 0.0005) and <50 years vs. >80 years ( = 0.0001). The findings were similar but less significant in a smaller replication cohort (2-sided = 0.037 in 50-80 years vs. >80 years and 0.061 in <50 years vs. >80 years). Analysis of the allele frequencies in 5-year bins demonstrated that the decrease of rs139185008C started after the age of 70 years. The hexanucleotide repeat expansion tagging single nucleotide polymorphisms decreasing frequency with age suggests its' association with age-related diseases probably also outside amyotrophic lateral sclerosis / frontotemporal dementia.