Hifumi Tatsuro, Tanaka Tetsuya, Sato Miho, Akioka Kohei, Fujimata Chiaki, Miyoshi Noriaki
Laboratory of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Vet Anim Sci. 2023 Mar 2;20:100291. doi: 10.1016/j.vas.2023.100291. eCollection 2023 Jun.
Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 () gene of for the rapid detection of equine alveolar echinococcosis. Thirty-six hepatic solid nodules obtained from each horse ( = 36) were evaluated based on histopathological examination and -targeted PCR and then submitted to the RPA assay. The results of the developed RPA assay were 94.4% consistent with those of PCR and Cohen's kappa coefficient value was 0.89 statistically, indicating high agreement. In addition, the RPA assay using the plasmid samples was one hundredfold more sensitive than PCR testing. Consequently, these results suggest that the performance of the RPA assay developed in this study is equal to that of conventional PCR testing.
屠宰马匹中的肺泡型棘球蚴病仍然是一个公共卫生问题。本研究旨在开发一种针对线粒体烟酰胺腺嘌呤二核苷酸脱氢酶亚基5（）基因的重组酶聚合酶扩增（RPA）检测方法，用于快速检测马肺泡型棘球蚴病。从每匹马（ = 36）获取的36个肝脏实性结节，基于组织病理学检查和靶向PCR进行评估，然后进行RPA检测。所开发的RPA检测结果与PCR结果的一致性为94.4%，Cohen卡方系数值在统计学上为0.89，表明高度一致。此外，使用质粒样本的RPA检测比PCR检测灵敏100倍。因此，这些结果表明本研究中开发的RPA检测性能与传统PCR检测相当。
