Fushimi Taiki, Oyama Shiori, Koizumi Ryo, Fujii Yasuyuki, Osakabe Naomi
Functional Control Systems, Graduate School of Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minumaku, Saitama 337-8570, Japan.
Department of Bioscience and Engineering, Shibaura Institute of Technology, Saitama, Saitama, Japan.
J Clin Biochem Nutr. 2023 Mar;72(2):132-138. doi: 10.3164/jcbn.22-50. Epub 2022 Dec 10.
Cyanidin 3--glucoside (C3G), an antioxidant, is one of the most abundant anthocyanin in plant foods. Intervention trials and subsequent meta-analyses have suggested that anthocyanins could reduce the risks of cardiovascular diseases. This study investigated hemodynamic alterations following a single intragastric dose of C3G by measuring blood flow in rat cremaster muscle arteriole for 60 min. Next, in excised aortas, we performed western blotting to measure the phosphorylation of Akt and endothelial nitric oxide synthase (eNOS). A single oral dose of C3G significantly increased blood flow soon after ingestion, and it was maintained throughout the experimental period. In addition, aortic Akt phosphorylation increased. Then, we examined the impact of repeated oral administrations of C3G for 14 days. The mean blood pressure was significantly reduced at 7 and 14 days after treatment, with a slight increase in aortic eNOS expression. Immunohistochemical analyses of the soleus showed that the level of CD31, an angiogenesis-marker protein, was significantly increased with C3G. These results suggested that an oral dose of C3G increased blood flow, which promoted angiogenesis within skeletal muscle, and consequently, blood pressure was reduced.
矢车菊素 3 - 葡萄糖苷（C3G）是一种抗氧化剂，是植物性食物中含量最为丰富的花青素之一。干预试验及后续的荟萃分析表明，花青素可降低心血管疾病风险。本研究通过测量大鼠提睾肌小动脉60分钟的血流量，探究单次胃内给予C3G后的血流动力学变化。接下来，在离体主动脉中，我们进行蛋白质免疫印迹法以检测Akt和内皮型一氧化氮合酶（eNOS）的磷酸化水平。单次口服C3G后，摄入不久血流量即显著增加，并在整个实验期间保持该状态。此外，主动脉Akt磷酸化增加。然后，我们检测了连续14天重复口服C3G的影响。治疗后第7天和第14天平均血压显著降低，主动脉eNOS表达略有增加。比目鱼肌的免疫组织化学分析显示，血管生成标记蛋白CD31的水平在C3G作用下显著升高。这些结果表明，口服C3G可增加血流量，促进骨骼肌内血管生成，进而降低血压。
