Ji Chihai, Zeng Meng, Wei Yingfang, Lv Xiaocheng, Sun Yuan, Ma Jingyun
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2023 Mar 1;14:1126707. doi: 10.3389/fmicb.2023.1126707. eCollection 2023.
In this study, we detected a circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus [named Po-Circo-like (PCL) virus] in intestinal tissue and fecal samples of pigs. PCL virus contains a single-stranded DNA genome, and ORF1 encodes the Rep and not the typical capsid protein encoded in PCV. The Rep protein may be responsible for viral genome replication. In addition, PCL virus may be one of the pathogens causing diarrhea symptoms in pigs. We identified four strains of PCL virus in two different pig farms with severe diarrhea outbreaks in Hunan Province, China. The strains in this study share 85.7-99.7% nucleic acid identity and 84.7-100% amino acid identity with Rep of the reference strains. A multiple sequence alignment of these PCL viruses and Bo-Circo-like CH showed a identity of 93.2% for the Rep protein, and the nucleotide identity was 86.7-89.3%. Moreover, Bo-Circo-like CH and HN75, HN39-01, HN39-02 had similar stem-loop sequences. In conclusion, the present study is the first detailed report of the PCL virus in Hunan provinces, which is a potential new virus in pigs that might be involved in cross-species transmission. Further investigation is needed to determine the pathogenesis of this virus and its epidemiologic impact.
在本研究中，我们在猪的肠道组织和粪便样本中检测到一种环状复制相关蛋白（Rep）编码的单链（CRESS）DNA病毒[命名为猪圆环样（PCL）病毒]。PCL病毒含有单链DNA基因组，开放阅读框1（ORF1）编码Rep，而非猪圆环病毒（PCV）中编码的典型衣壳蛋白。Rep蛋白可能负责病毒基因组的复制。此外，PCL病毒可能是导致猪腹泻症状的病原体之一。我们在中国湖南省两个腹泻疫情严重的不同猪场中鉴定出四株PCL病毒。本研究中的毒株与参考毒株的Rep在核酸水平上具有85.7% - 99.7%的同一性，在氨基酸水平上具有84.7% - 100%的同一性。这些PCL病毒与博卡圆环样CH毒株的多序列比对显示，Rep蛋白的同一性为93.2%，核苷酸同一性为86.7% - 89.3%。此外，博卡圆环样CH与HN75、HN39 - 01、HN39 - 02具有相似的茎环序列。总之，本研究是湖南省关于PCL病毒的首次详细报道，该病毒是猪中一种可能参与跨物种传播的潜在新病毒。需要进一步研究以确定该病毒的发病机制及其流行病学影响。
