Green Joseph M, Barry Fatoumata, Burton Phoebe, Beauchemin Jennifer, Huentelman Matthew J, Deoni Sean C L, Lewis Candace R
School of Life Sciences, Arizona State University, Tempe, AZ, United States.
Rhode Island Hospital, Warren Alpert Medical School, Brown University, Providence, RI, United States.
Front Pediatr. 2023 Mar 3;11:1059107. doi: 10.3389/fped.2023.1059107. eCollection 2023.
Self-collection of dried blood samples (DBS) in the participant's home provides an alternative to university/hospital visits for research and has the potential to improve the representation of population heterogeneity in research. This study aimed to assess the feasibility of guardian and/or self-DBS collection in healthy youth in the lab and home. Guardians/youth [ = 140; females = 63; = 8.73, = 3.56] who enrolled in a longitudinal study of typical development were asked during a lab visit to provide a DBS. Upon providing a sample, the participants were asked if they would be willing to self-collect in the home and return the sample the post office. Of those asked to provide a sample in the lab, 82% consented and 18% declined, with a significant difference in age but no significant difference in sex, ethnicity, race, or family income. Of those who provided a sample in the lab, 75% were willing to self-collect DBS in the home, with no significant difference in demographic variables between them. We report a quality assessment and DNA extraction results from a subset of samples. The results demonstrate a high feasibility of DBS collection from healthy youth for research purposes both in the laboratory and in the home across different demographic variables. Developmental researchers should consider including this approach in their studies to increase population heterogeneity representation.
让参与者在家自行采集干血斑样本（DBS）为研究提供了一种替代去大学/医院就诊的方式，并且有可能改善研究中人群异质性的代表性。本研究旨在评估在实验室和家中由监护人及/或自行采集DBS样本对于健康青少年的可行性。参与一项典型发育纵向研究的监护人/青少年[=140；女性=63；=8.73，=3.56]在实验室访视期间被要求提供一份DBS样本。在提供样本后，参与者被询问是否愿意在家自行采集样本并将其寄回邮局。在那些被要求在实验室提供样本的人中，82%同意，18%拒绝，年龄存在显著差异，但在性别、种族、族裔或家庭收入方面无显著差异。在那些在实验室提供样本的人中，75%愿意在家自行采集DBS样本，并在人口统计学变量方面无显著差异。我们报告了一部分样本的质量评估和DNA提取结果。结果表明，从健康青少年中采集DBS样本用于研究目的，无论是在实验室还是在家中，跨越不同人口统计学变量都具有很高的可行性。发育研究人员应考虑在其研究中纳入这种方法，以增加人群异质性的代表性。
